2x8a

Human Nuclear Valosin containing protein Like (NVL), C-terminal AAA- ATPase domainHuman Nuclear Valosin containing protein Like (NVL), C-terminal AAA- ATPase domain

Structural highlights

2x8a is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NVL_HUMAN Participates in the assembly of the telomerase holoenzyme and effecting of telomerase activity via its interaction with TERT (PubMed:22226966). Involved in both early and late stages of the pre-rRNA processing pathways (PubMed:26166824). Spatiotemporally regulates 60S ribosomal subunit biogenesis in the nucleolus (PubMed:15469983, PubMed:16782053, PubMed:29107693, PubMed:26456651). Catalyzes the release of specific assembly factors, such as WDR74, from pre-60S ribosomal particles through the ATPase activity (PubMed:29107693, PubMed:26456651, PubMed:28416111).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Nagahama M, Hara Y, Seki A, Yamazoe T, Kawate Y, Shinohara T, Hatsuzawa K, Tani K, Tagaya M. NVL2 is a nucleolar AAA-ATPase that interacts with ribosomal protein L5 through its nucleolar localization sequence. Mol Biol Cell. 2004 Dec;15(12):5712-23. doi: 10.1091/mbc.e04-08-0692. Epub 2004, Oct 6. PMID:15469983 doi:http://dx.doi.org/10.1091/mbc.e04-08-0692
↑ Nagahama M, Yamazoe T, Hara Y, Tani K, Tsuji A, Tagaya M. The AAA-ATPase NVL2 is a component of pre-ribosomal particles that interacts with the DExD/H-box RNA helicase DOB1. Biochem Biophys Res Commun. 2006 Aug 4;346(3):1075-82. doi:, 10.1016/j.bbrc.2006.06.017. Epub 2006 Jun 12. PMID:16782053 doi:http://dx.doi.org/10.1016/j.bbrc.2006.06.017
↑ Her J, Chung IK. The AAA-ATPase NVL2 is a telomerase component essential for holoenzyme assembly. Biochem Biophys Res Commun. 2012 Jan 20;417(3):1086-92. doi:, 10.1016/j.bbrc.2011.12.101. Epub 2011 Dec 27. PMID:22226966 doi:http://dx.doi.org/10.1016/j.bbrc.2011.12.101
↑ Yoshikatsu Y, Ishida Y, Sudo H, Yuasa K, Tsuji A, Nagahama M. NVL2, a nucleolar AAA-ATPase, is associated with the nuclear exosome and is involved in pre-rRNA processing. Biochem Biophys Res Commun. 2015 Aug 28;464(3):780-6. doi:, 10.1016/j.bbrc.2015.07.032. Epub 2015 Jul 10. PMID:26166824 doi:http://dx.doi.org/10.1016/j.bbrc.2015.07.032
↑ Hiraishi N, Ishida Y, Nagahama M. AAA-ATPase NVL2 acts on MTR4-exosome complex to dissociate the nucleolar protein WDR74. Biochem Biophys Res Commun. 2015 Nov 20;467(3):534-40. doi:, 10.1016/j.bbrc.2015.09.160. Epub 2015 Oct 8. PMID:26456651 doi:http://dx.doi.org/10.1016/j.bbrc.2015.09.160
↑ Lo YH, Romes EM, Pillon MC, Sobhany M, Stanley RE. Structural Analysis Reveals Features of Ribosome Assembly Factor Nsa1/WDR74 Important for Localization and Interaction with Rix7/NVL2. Structure. 2017 May 2;25(5):762-772.e4. doi: 10.1016/j.str.2017.03.008. Epub 2017, Apr 13. PMID:28416111 doi:http://dx.doi.org/10.1016/j.str.2017.03.008
↑ Hiraishi N, Ishida YI, Sudo H, Nagahama M. WDR74 participates in an early cleavage of the pre-rRNA processing pathway in cooperation with the nucleolar AAA-ATPase NVL2. Biochem Biophys Res Commun. 2018 Jan 1;495(1):116-123. doi:, 10.1016/j.bbrc.2017.10.148. Epub 2017 Oct 28. PMID:29107693 doi:http://dx.doi.org/10.1016/j.bbrc.2017.10.148

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OCA

[1] Nagahama M, Hara Y, Seki A, Yamazoe T, Kawate Y, Shinohara T, Hatsuzawa K, Tani K, Tagaya M. NVL2 is a nucleolar AAA-ATPase that interacts with ribosomal protein L5 through its nucleolar localization sequence. Mol Biol Cell. 2004 Dec;15(12):5712-23. doi: 10.1091/mbc.e04-08-0692. Epub 2004, Oct 6. PMID:15469983 doi:http://dx.doi.org/10.1091/mbc.e04-08-0692

[2] Nagahama M, Yamazoe T, Hara Y, Tani K, Tsuji A, Tagaya M. The AAA-ATPase NVL2 is a component of pre-ribosomal particles that interacts with the DExD/H-box RNA helicase DOB1. Biochem Biophys Res Commun. 2006 Aug 4;346(3):1075-82. doi:, 10.1016/j.bbrc.2006.06.017. Epub 2006 Jun 12. PMID:16782053 doi:http://dx.doi.org/10.1016/j.bbrc.2006.06.017

[3] Her J, Chung IK. The AAA-ATPase NVL2 is a telomerase component essential for holoenzyme assembly. Biochem Biophys Res Commun. 2012 Jan 20;417(3):1086-92. doi:, 10.1016/j.bbrc.2011.12.101. Epub 2011 Dec 27. PMID:22226966 doi:http://dx.doi.org/10.1016/j.bbrc.2011.12.101

[4] Yoshikatsu Y, Ishida Y, Sudo H, Yuasa K, Tsuji A, Nagahama M. NVL2, a nucleolar AAA-ATPase, is associated with the nuclear exosome and is involved in pre-rRNA processing. Biochem Biophys Res Commun. 2015 Aug 28;464(3):780-6. doi:, 10.1016/j.bbrc.2015.07.032. Epub 2015 Jul 10. PMID:26166824 doi:http://dx.doi.org/10.1016/j.bbrc.2015.07.032

[5] Hiraishi N, Ishida Y, Nagahama M. AAA-ATPase NVL2 acts on MTR4-exosome complex to dissociate the nucleolar protein WDR74. Biochem Biophys Res Commun. 2015 Nov 20;467(3):534-40. doi:, 10.1016/j.bbrc.2015.09.160. Epub 2015 Oct 8. PMID:26456651 doi:http://dx.doi.org/10.1016/j.bbrc.2015.09.160

[6] Lo YH, Romes EM, Pillon MC, Sobhany M, Stanley RE. Structural Analysis Reveals Features of Ribosome Assembly Factor Nsa1/WDR74 Important for Localization and Interaction with Rix7/NVL2. Structure. 2017 May 2;25(5):762-772.e4. doi: 10.1016/j.str.2017.03.008. Epub 2017, Apr 13. PMID:28416111 doi:http://dx.doi.org/10.1016/j.str.2017.03.008

[7] Hiraishi N, Ishida YI, Sudo H, Nagahama M. WDR74 participates in an early cleavage of the pre-rRNA processing pathway in cooperation with the nucleolar AAA-ATPase NVL2. Biochem Biophys Res Commun. 2018 Jan 1;495(1):116-123. doi:, 10.1016/j.bbrc.2017.10.148. Epub 2017 Oct 28. PMID:29107693 doi:http://dx.doi.org/10.1016/j.bbrc.2017.10.148

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