6iew

The crystal structure of the dNxf2 UBA domain in complex with PanoramixThe crystal structure of the dNxf2 UBA domain in complex with Panoramix

Structural highlights

6iew is a 1 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.5Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NXF2_DROME May be involved in the export of mRNA from the nucleus to the cytoplasm.^[1] PANX_DROME Acts via the piwi-interacting RNA (piRNA) pathway which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:26472911, PubMed:26494711). Required for transcriptional silencing of transposons targeted by piwi and confers its effects by interacting with nascent RNA transcripts (PubMed:26472911, PubMed:26494711). Likely to be recruited to nascent transcripts cotranscriptionally by piwi and to recruit additional factors involved in transcriptional silencing (PubMed:26472911). In the ovaries, forms a complex with nxf2, piwi and Nxt1 which acts as effectors of cotranscriptional transposon silencing (PubMed:31219034, PubMed:31368590, PubMed:31570835, PubMed:31384064). The interaction with nxf2 stabilizes the nuclear protein complex (PubMed:31384064).^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

References

↑ Herold A, Klymenko T, Izaurralde E. NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila. RNA. 2001 Dec;7(12):1768-80. PMID:11780633
↑ Yu Y, Gu J, Jin Y, Luo Y, Preall JB, Ma J, Czech B, Hannon GJ. Panoramix enforces piRNA-dependent cotranscriptional silencing. Science. 2015 Oct 16;350(6258):339-42. PMID:26472911 doi:10.1126/science.aab0700
↑ Sienski G, Batki J, Senti KA, Dönertas D, Tirian L, Meixner K, Brennecke J. Silencio/CG9754 connects the Piwi-piRNA complex to the cellular heterochromatin machinery. Genes Dev. 2015 Nov 1;29(21):2258-71. PMID:26494711 doi:10.1101/gad.271908.115
↑ Fabry MH, Ciabrelli F, Munafò M, Eastwood EL, Kneuss E, Falciatori I, Falconio FA, Hannon GJ, Czech B. piRNA-guided co-transcriptional silencing coopts nuclear export factors. Elife. 2019 Jun 20;8:e47999. PMID:31219034 doi:10.7554/eLife.47999
↑ Murano K, Iwasaki YW, Ishizu H, Mashiko A, Shibuya A, Kondo S, Adachi S, Suzuki S, Saito K, Natsume T, Siomi MC, Siomi H. Nuclear RNA export factor variant initiates piRNA-guided co-transcriptional silencing. EMBO J. 2019 Sep 2;38(17):e102870. PMID:31368590 doi:10.15252/embj.2019102870
↑ Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J. The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. Nat Struct Mol Biol. 2019 Aug;26(8):720-731. doi: 10.1038/s41594-019-0270-6. Epub, 2019 Aug 5. PMID:31384064 doi:http://dx.doi.org/10.1038/s41594-019-0270-6
↑ Zhao K, Cheng S, Miao N, Xu P, Lu X, Zhang Y, Wang M, Ouyang X, Yuan X, Liu W, Lu X, Zhou P, Gu J, Zhang Y, Qiu D, Jin Z, Su C, Peng C, Wang JH, Dong MQ, Wan Y, Ma J, Cheng H, Huang Y, Yu Y. A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation. Nat Cell Biol. 2019 Oct;21(10):1261-1272. doi: 10.1038/s41556-019-0396-0. Epub, 2019 Sep 30. PMID:31570835 doi:http://dx.doi.org/10.1038/s41556-019-0396-0

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OCA

[1] Herold A, Klymenko T, Izaurralde E. NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila. RNA. 2001 Dec;7(12):1768-80. PMID:11780633

[2] Yu Y, Gu J, Jin Y, Luo Y, Preall JB, Ma J, Czech B, Hannon GJ. Panoramix enforces piRNA-dependent cotranscriptional silencing. Science. 2015 Oct 16;350(6258):339-42. PMID:26472911 doi:10.1126/science.aab0700

[3] Sienski G, Batki J, Senti KA, Dönertas D, Tirian L, Meixner K, Brennecke J. Silencio/CG9754 connects the Piwi-piRNA complex to the cellular heterochromatin machinery. Genes Dev. 2015 Nov 1;29(21):2258-71. PMID:26494711 doi:10.1101/gad.271908.115

[4] Fabry MH, Ciabrelli F, Munafò M, Eastwood EL, Kneuss E, Falciatori I, Falconio FA, Hannon GJ, Czech B. piRNA-guided co-transcriptional silencing coopts nuclear export factors. Elife. 2019 Jun 20;8:e47999. PMID:31219034 doi:10.7554/eLife.47999

[5] Murano K, Iwasaki YW, Ishizu H, Mashiko A, Shibuya A, Kondo S, Adachi S, Suzuki S, Saito K, Natsume T, Siomi MC, Siomi H. Nuclear RNA export factor variant initiates piRNA-guided co-transcriptional silencing. EMBO J. 2019 Sep 2;38(17):e102870. PMID:31368590 doi:10.15252/embj.2019102870

[6] Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J. The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. Nat Struct Mol Biol. 2019 Aug;26(8):720-731. doi: 10.1038/s41594-019-0270-6. Epub, 2019 Aug 5. PMID:31384064 doi:http://dx.doi.org/10.1038/s41594-019-0270-6

[7] Zhao K, Cheng S, Miao N, Xu P, Lu X, Zhang Y, Wang M, Ouyang X, Yuan X, Liu W, Lu X, Zhou P, Gu J, Zhang Y, Qiu D, Jin Z, Su C, Peng C, Wang JH, Dong MQ, Wan Y, Ma J, Cheng H, Huang Y, Yu Y. A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation. Nat Cell Biol. 2019 Oct;21(10):1261-1272. doi: 10.1038/s41556-019-0396-0. Epub, 2019 Sep 30. PMID:31570835 doi:http://dx.doi.org/10.1038/s41556-019-0396-0

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