3kg4

Crystal structure of an uncharacterized protein from Mannheimia succiniciproducensCrystal structure of an uncharacterized protein from Mannheimia succiniciproducens

Structural highlights 3kg4 is a 1 chain structure with sequence from Mannheimia succiniciproducens MBEL55E. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.95Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CAS5D_MANSM CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein is a sequence-specific endonuclease that cleaves pre-crRNA at G21 into mature crRNA. Does not cleave pre-crRNA associated with the T.thermophilus strain HB27 Cas5 protein (AC Q746C2) CRISPR locus (PubMed:23006625). The reaction mechanism may proceed by an intramolecular attack of the 2'-hydroxyl group of G21 on the scissile phosphodiester, cutting the precursor 3' to G21 residue yielding 5'-hydroxyl and 2' and/or 3' ends lacking a hydroxyl group (perhaps a 2'/3' cyclic phosphodiester) (Ref.2). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. See Also CRISPR subtype I-C CRISPR-Cas

spinqualitylabelsall models 3kg4, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image