3j1a

HK97-like fold fitted into 3D reconstruction of bacteriophage CW02HK97-like fold fitted into 3D reconstruction of bacteriophage CW02

3j1a, resolution 16.00Å Show:Asymmetric Unit Biological Assembly Structural highlights 3j1a is a 7 chain structure with sequence from Unidentified. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 16Å Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CAPSD_BPHK7 Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function. Publication Abstract from PubMed Halophage CW02 infects a Salinivibrio costicola-like bacterium, SA50, isolated from the Great Salt Lake. Following isolation, cultivation, and purification, CW02 was characterized by DNA sequencing, mass spectrometry, and electron microscopy. A conserved module of structural genes places CW02 in the T7 supergroup, members of which are found in diverse aquatic environments, including marine and freshwater ecosystems. CW02 has morphological similarities to viruses of the Podoviridae family. The structure of CW02, solved by cryogenic electron microscopy (cryo-EM) and three-dimensional reconstruction, enabled fitting of a portion of the bacteriophage HK97 capsid protein into CW02 capsid density, thereby providing additional evidence that capsid proteins of dsDNA tailed phages have a conserved fold. The CW02 capsid consists of bacteriophage lambda gpD-like densities that likely contribute to particle stability. Turret-like densities were found on icosahedral vertices and may represent a unique adaptation similar to what has been seen in other extremophilic viruses that infect archaea, such as Sulfolobus turreted icosahedral virus and halophage SH1. Sequence and Structural Characterization of Great Salt Lake Bacteriophage CW02, a Member of the T7-like Supergroup.,Shen PS, Domek MJ, Sanz-Garcia E, Makaju A, Taylor RM, Hoggan R, Culumber M, Oberg C, Breakwell DP, Prince JT, Belnap DM J Virol. 2012 May 16. PMID:22593163 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.