1rg8
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Human Acidic Fibroblast Growth Factor (haFGF-1) at 1.10 angstrom resolution (140 amino acid form)
OverviewOverview
A 1.10-A atomic resolution X-ray structure of human fibroblast growth, factor 1 (FGF-1), a member of the beta-trefoil superfold, has been, determined. The beta-trefoil is one of 10 fundamental protein superfolds, and is the only superfold to exhibit 3-fold structural symmetry, (comprising 3 "trefoil" units). The quality of the diffraction data, permits unambiguous assignment of Asn, Gln, and His rotamers, Pro ring, pucker, as well as refinement of atomic anisotropic displacement, parameters (ADPs). The FGF-1 structure exhibits numerous core-packing, defects, detectable using a 1.0-A probe radius. In addition to, contributing to the relatively low thermal stability of FGF-1, these, defects may also permit domain motions within the structure. The, availability of refined ADPs allows a translation/libration/screw (TLS), analysis of putative rigid body domains. The TLS analysis shows that, beta-strands 6-12 together form a rigid body, and there is a clear, demarcation in TLS motions between the adjacent carboxyl- and, amino-termini. Although separate from beta-strands 6-12, the individual, beta-strands 1-5 do not exhibit correlated motions; thus, this region, appears to be comparatively flexible. The heparin-binding contacts of, FGF-1 are located within beta-strands 6-12; conversely, a significant, portion of the receptor-binding contacts are located within beta-strands, 1-5. Thus, the observed rigid body motion in FGF-1 appears related to the, ligand-binding functionalities.
DiseaseDisease
Known diseases associated with this structure: Aplasia of lacrimal and salivary glands OMIM:[602115], LADD syndrome OMIM:[602115]
About this StructureAbout this Structure
1RG8 is a Single protein structure of sequence from Homo sapiens with FMT as ligand. Full crystallographic information is available from OCA.
ReferenceReference
An atomic resolution structure for human fibroblast growth factor 1., Bernett MJ, Somasundaram T, Blaber M, Proteins. 2004 Nov 15;57(3):626-34. PMID:15382229
Page seeded by OCA on Mon Nov 12 19:03:16 2007