1rf1
Crystal Structure of Fragment D of gammaE132A Fibrinogen with the Peptide Ligand Gly-His-Arg-Pro-amide
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OverviewOverview
Structural analysis of recombinant fibrinogen fragment D revealed that the, calcium-binding site (beta2-site) composed of residues BbetaAsp261, BbetaAsp398, BbetaGly263, and gammaGlu132 is modulated by the "B:b", interaction. To determine the beta2-site's role in polymerization, we, engineered variant fibrinogen gammaE132A in which calcium binding to the, beta2-site was disrupted by replacing glutamic acid at gamma132 with, alanine. We compared polymerization of gammaE132A to normal fibrinogen as, a function of calcium concentration. Polymerization of gammaE132A at, concentrations of calcium <or=1 mM exhibited an uncharacteristic 2-3-fold, increase in lateral aggregation and fiber thickness compared to normal, fibrinogen, while polymerization of variant and normal were, indistinguishable at 10 mM calcium. These results suggest that the, beta2-site controls the extent of lateral aggregation. That is, when the, calcium anchor (beta2-site) is eliminated before "B:b" interactions occur, then lateral aggregation is enhanced. We solved structures of fragment D, of gammaE132A fibrinogen (rfD-gammaE132A) with and without, Gly-His-Arg-Pro-amide (GHRPam) and found no change to the global, structure. X-ray diffraction data showed GHRPam binding in the "a" and "b", polymerization sites and that calcium could still bind to the beta2-site, of gammaE132A fibrinogen at 70 mM calcium. We found that the gamma2, calcium-binding site (in loop gamma294-301) did not have calcium bound in, the structure of fragment D of gammaE132A fibrinogen with GHRPam bound, (rfD-gammaE132A+GH). Analysis of structures rfD-gammaE132A+GH and, rfD-BbetaD398A+GH indicated that differences in calcium occupation of the, gamma2-site resulted from minor conformational changes provoked by crystal, packing and GHRPam binding to the "a" site did not directly modulate, calcium binding to this site.
DiseaseDisease
Known diseases associated with this structure: Afibrinogenemia, congenital OMIM:[134820], Afibrinogenemia, congenital OMIM:[134830], Amyloidosis, hereditary renal OMIM:[134820], Dysfibrinogenemia, alpha type, causing bleeding diathesis OMIM:[134820], Dysfibrinogenemia, alpha type, causing recurrent thrombosis OMIM:[134820], Dysfibrinogenemia, beta type OMIM:[134830], Dysfibrinogenemia, gamma type OMIM:[134850], Hypofibrinogenemia, gamma type OMIM:[134850], Thrombophilia, dysfibrinogenemic OMIM:[134830], Thrombophilia, dysfibrinogenemic OMIM:[134850]
About this StructureAbout this Structure
1RF1 is a Protein complex structure of sequences from Homo sapiens with CA as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Calcium-binding site beta 2, adjacent to the "b" polymerization site, modulates lateral aggregation of protofibrils during fibrin polymerization., Kostelansky MS, Lounes KC, Ping LF, Dickerson SK, Gorkun OV, Lord ST, Biochemistry. 2004 Mar 9;43(9):2475-83. PMID:14992585
Page seeded by OCA on Mon Nov 12 19:02:42 2007