2xkm

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Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMRConsensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR

Structural highlights

2xkm is a 1 chain structure with sequence from Pseudomonas virus Pf1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Fiber diffraction , Hybrid , Solid-state NMR, Resolution 3.3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAPSD_BPPF1 Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).

Publication Abstract from PubMed

Filamentous bacteriophages (filamentous bacterial viruses or Inovirus) are simple and well-characterised macromolecular assemblies that are widely used in molecular biology and biophysics, both as paradigms for studying basic biological questions and as practical tools in areas as diverse as immunology and solid-state physics. The strains fd, M13 and f1 are virtually identical filamentous phages that infect bacteria expressing F-pili, and are sometimes grouped as the Ff phages. For historical reasons fd has often been used for structural studies, but M13 and f1 are more often used for biological experiments. Many other strains have been identified that are genetically quite distinct from Ff and yet have a similar molecular structure and life cycle. One of these, Pf1, gives the highest resolution X-ray fibre diffraction patterns known for filamentous bacteriophage. These diffraction patterns have been used in the past to derive a molecular model for the structure of the phage. Solid-state NMR experiments have been used in separate studies to derive a significantly different model of Pf1. Here we combine previously published X-ray fibre diffraction data and solid-state NMR data to give a consensus structure model for Pf1 filamentous bacteriophage, and we discuss the implications of this model for assembly of the phage at the bacterial membrane.

Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR.,Straus SK, Scott WR, Schwieters CD, Marvin DA Eur Biophys J. 2010 Nov 17. PMID:21082179[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Straus SK, Scott WR, Schwieters CD, Marvin DA. Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR. Eur Biophys J. 2010 Nov 17. PMID:21082179 doi:10.1007/s00249-010-0640-9

2xkm, resolution 3.30Å

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