2b9z

Solution structure of FHV B2, a viral suppressor of RNAiSolution structure of FHV B2, a viral suppressor of RNAi

Structural highlights

2b9z is a 2 chain structure with sequence from Flock House virus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

B2_FHV Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response.^[1] ^[2]

Publication Abstract from PubMed

We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three alpha-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (alpha2/alpha2') forms an elongated binding interface with double-stranded RNA (dsRNA). This implies a novel mode of dsRNA recognition and provides insights into the mechanism of RNAi suppression by B2.

The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition.,Lingel A, Simon B, Izaurralde E, Sattler M EMBO Rep. 2005 Dec;6(12):1149-55. PMID:16270100^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Li WX, Li H, Lu R, Li F, Dus M, Atkinson P, Brydon EW, Johnson KL, Garcia-Sastre A, Ball LA, Palese P, Ding SW. Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing. Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1350-5. Epub 2004 Jan 26. PMID:14745017 doi:http://dx.doi.org/10.1073/pnas.0308308100
↑ Chao JA, Lee JH, Chapados BR, Debler EW, Schneemann A, Williamson JR. Dual modes of RNA-silencing suppression by Flock House virus protein B2. Nat Struct Mol Biol. 2005 Nov;12(11):952-7. PMID:16228003 doi:10.1038/nsmb1005
↑ Lingel A, Simon B, Izaurralde E, Sattler M. The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition. EMBO Rep. 2005 Dec;6(12):1149-55. PMID:16270100

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OCA

[1] Li WX, Li H, Lu R, Li F, Dus M, Atkinson P, Brydon EW, Johnson KL, Garcia-Sastre A, Ball LA, Palese P, Ding SW. Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing. Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1350-5. Epub 2004 Jan 26. PMID:14745017 doi:http://dx.doi.org/10.1073/pnas.0308308100

[2] Chao JA, Lee JH, Chapados BR, Debler EW, Schneemann A, Williamson JR. Dual modes of RNA-silencing suppression by Flock House virus protein B2. Nat Struct Mol Biol. 2005 Nov;12(11):952-7. PMID:16228003 doi:10.1038/nsmb1005

[3] Lingel A, Simon B, Izaurralde E, Sattler M. The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition. EMBO Rep. 2005 Dec;6(12):1149-55. PMID:16270100

[1]

[2]

[3]

2b9z

Solution structure of FHV B2, a viral suppressor of RNAiSolution structure of FHV B2, a viral suppressor of RNAi

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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2b9z

Solution structure of FHV B2, a viral suppressor of RNAiSolution structure of FHV B2, a viral suppressor of RNAi

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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