5o2y

NMR structure of the calcium bound form of PulG, major pseudopilin from Klebsiella oxytoca T2SSNMR structure of the calcium bound form of PulG, major pseudopilin from Klebsiella oxytoca T2SS

Structural highlights 5o2y is a 1 chain structure with sequence from Klebsiella oxytoca. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Ligands: Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Many Gram-negative bacteria use type 2 secretion systems (T2SSs) to secrete proteins involved in virulence and adaptation. Transport of folded proteins via T2SS nanomachines requires the assembly of inner membrane-anchored fibres called pseudopili. Although efficient pseudopilus assembly is essential for protein secretion, structure-based functional analyses are required to unravel the mechanistic link between these processes. Here, we report an atomic model for a T2SS pseudopilus from Klebsiella oxytoca, obtained by fitting the NMR structure of its calcium-bound subunit PulG into the ~5-A-resolution cryo-electron microscopy reconstruction of assembled fibres. This structure reveals the comprehensive network of inter-subunit contacts and unexpected features, including a disordered central region of the PulG helical stem, and highly flexible C-terminal residues on the fibre surface. NMR, mutagenesis and functional analyses highlight the key role of calcium in PulG folding and stability. Fibre disassembly in the absence of calcium provides a basis for pseudopilus length control, essential for protein secretion, and supports the Archimedes screw model for the type 2 secretion mechanism.An atomic model for a type 2 secretion system pseudopilus from Klebsiella oxytoca reveals a comprehensive network of inter-subunit contacts while mutational and functional analyses highlight the role of calcium in PulG folding and stability. Structure of the calcium-dependent type 2 secretion pseudopilus.,Lopez-Castilla A, Thomassin JL, Bardiaux B, Zheng W, Nivaskumar M, Yu X, Nilges M, Egelman EH, Izadi-Pruneyre N, Francetic O Nat Microbiol. 2017 Oct 9. doi: 10.1038/s41564-017-0041-2. PMID:28993624 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Pseudopilin

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