1puf
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Crystal Structure of HoxA9 and Pbx1 homeodomains bound to DNA
OverviewOverview
The HOX/HOM superfamily of homeodomain proteins controls cell fate and, segmental embryonic patterning by a mechanism that is conserved in all, metazoans. The linear arrangement of the Hox genes on the chromosome, correlates with the spatial distribution of HOX protein expression along, the anterior-posterior axis of the embryo. Most HOX proteins bind DNA, cooperatively with members of the PBC family of TALE-type homeodomain, proteins, which includes human Pbx1. Cooperative DNA binding between HOX, and PBC proteins requires a residue N-terminal to the HOX homeodomain, termed the hexapeptide, which differs significantly in sequence between, anterior- and posterior-regulating HOX proteins. We report here the, 1.9-A-resolution structure of a posterior HOX protein, HoxA9, complexed, with Pbx1 and DNA, which reveals that the posterior Hox hexapeptide adopts, an altered conformation as compared with that seen in previously, determined anterior HOX/PBC structures. The additional nonspecific, interactions and altered DNA conformation in this structure account for, the stronger DNA-binding affinity and altered specificity observed for, posterior HOX proteins when compared with anterior HOX proteins., DNA-binding studies of wild-type and mutant HoxA9 and HoxB1 show residues, in the N-terminal arm of the homeodomains are critical for proper DNA, sequence recognition despite lack of direct contact by these residues to, the DNA bases. These results help shed light on the mechanism of, transcriptional regulation by HOX proteins and show how DNA-binding, proteins may use indirect contacts to determine sequence specificity.
DiseaseDisease
Known diseases associated with this structure: Colorectal cancer OMIM:[604584], Hepatocellular cancer OMIM:[604584], Leukemia, acute pre-B-cell OMIM:[176310]
About this StructureAbout this Structure
1PUF is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior., LaRonde-LeBlanc NA, Wolberger C, Genes Dev. 2003 Aug 15;17(16):2060-72. PMID:12923056
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