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Crystal structure of Arabidopsis thaliana HPPD complexed with 4-hydroxyphenylacetic acidCrystal structure of Arabidopsis thaliana HPPD complexed with 4-hydroxyphenylacetic acid
Structural highlights
FunctionPublication Abstract from PubMedIncreasing demands for efficient and versatile chemical reactions have prompted innovations in enzyme engineering. A major challenge in engineering alpha-ketoglutarate-dependent oxygenases is to develop a rational strategy which can be widely used for directly evolving the desired mutant to generate new products. Herein, we report a strategy for rational redesign of a model enzyme, 4-hydroxyphenylpyruvate dioxygenase (HPPD), based on quantum mechanics/molecular mechanics (QM/MM) calculation and molecular dynamic simulations. This strategy enriched our understanding of the HPPD catalytic reaction pathway and led to the discovery of a series of HPPD mutants producing hydroxyphenylacetate (HPA) as the alternative product other than the native product homogentisate. The predicted HPPD-Fe(IV) horizontal lineO-HPA intermediate was further confirmed by the crystal structure of Arabidopsis thaliana HPPD/S267W complexed with HPA. These findings not only provide a good understanding of the structure-function relationship of HPPD but also demonstrate a generally applicable platform for the development of biocatalysts. Rational Redesign of Enzyme via the Combination of Quantum Mechanics/Molecular Mechanics, Molecular Dynamics, and Structural Biology Study.,Lin HY, Chen X, Dong J, Yang JF, Xiao H, Ye Y, Li LH, Zhan CG, Yang WC, Yang GF J Am Chem Soc. 2021 Sep 29;143(38):15674-15687. doi: 10.1021/jacs.1c06227. Epub, 2021 Sep 20. PMID:34542283[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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