4c0k

Crystal structure of Drosophila Miro EF hand and cGTPase domains bound to one calcium ion (Ca-MiroS)Crystal structure of Drosophila Miro EF hand and cGTPase domains bound to one calcium ion (Ca-MiroS)

Structural highlights

4c0k is a 1 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.801Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MIRO_DROME Mitochondrial GTPase involved in mitochondrial trafficking. Milt and Miro form an essential protein complex that links Khc to mitochondria for light chain-independent, anterograde transport of mitochondria. Required for axonal transport to synapses within nerve terminals. Required presynaptically but not postsynaptically at neuromuscular junctions (NMJs).^[1] ^[2]

Publication Abstract from PubMed

Miro is a highly conserved calcium-binding GTPase at the regulatory nexus of mitochondrial transport and autophagy. Here we present crystal structures comprising the tandem EF hand and carboxy terminal GTPase (cGTPase) domains of Drosophila Miro. The structures reveal two previously unidentified 'hidden' EF hands, each paired with a canonical EF hand. Each EF hand pair is bound to a helix that structurally mimics an EF hand ligand. A key nucleotide-sensing element and a Pink1 phosphorylation site both lie within an extensive EF hand-cGTPase interface. Our results indicate structural mechanisms for calcium, nucleotide and phosphorylation-dependent regulation of mitochondrial function by Miro.

Structural coupling of the EF hand and C-terminal GTPase domains in the mitochondrial protein Miro.,Klosowiak JL, Focia PJ, Chakravarthy S, Landahl EC, Freymann DM, Rice SE EMBO Rep. 2013 Sep 27. doi: 10.1038/embor.2013.151. PMID:24071720^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Guo X, Macleod GT, Wellington A, Hu F, Panchumarthi S, Schoenfield M, Marin L, Charlton MP, Atwood HL, Zinsmaier KE. The GTPase dMiro is required for axonal transport of mitochondria to Drosophila synapses. Neuron. 2005 Aug 4;47(3):379-93. PMID:16055062 doi:http://dx.doi.org/10.1016/j.neuron.2005.06.027
↑ Glater EE, Megeath LJ, Stowers RS, Schwarz TL. Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent. J Cell Biol. 2006 May 22;173(4):545-57. PMID:16717129 doi:http://dx.doi.org/jcb.200601067
↑ Klosowiak JL, Focia PJ, Chakravarthy S, Landahl EC, Freymann DM, Rice SE. Structural coupling of the EF hand and C-terminal GTPase domains in the mitochondrial protein Miro. EMBO Rep. 2013 Sep 27. doi: 10.1038/embor.2013.151. PMID:24071720 doi:http://dx.doi.org/10.1038/embor.2013.151

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OCA

[1] Guo X, Macleod GT, Wellington A, Hu F, Panchumarthi S, Schoenfield M, Marin L, Charlton MP, Atwood HL, Zinsmaier KE. The GTPase dMiro is required for axonal transport of mitochondria to Drosophila synapses. Neuron. 2005 Aug 4;47(3):379-93. PMID:16055062 doi:http://dx.doi.org/10.1016/j.neuron.2005.06.027

[2] Glater EE, Megeath LJ, Stowers RS, Schwarz TL. Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent. J Cell Biol. 2006 May 22;173(4):545-57. PMID:16717129 doi:http://dx.doi.org/jcb.200601067

[3] Klosowiak JL, Focia PJ, Chakravarthy S, Landahl EC, Freymann DM, Rice SE. Structural coupling of the EF hand and C-terminal GTPase domains in the mitochondrial protein Miro. EMBO Rep. 2013 Sep 27. doi: 10.1038/embor.2013.151. PMID:24071720 doi:http://dx.doi.org/10.1038/embor.2013.151

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