2ji8
|
X-RAY STRUCTURE OF OXALYL-COA DECARBOXYLASE IN COMPLEX WITH FORMYL-COA
OverviewOverview
Despite more than five decades of extensive studies of thiamin diphosphate, (ThDP) enzymes, there remain many uncertainties as to how these enzymes, achieve their rate enhancements. Here, we present a clear picture of, catalysis for the simple nonoxidative decarboxylase, oxalyl-coenzyme A, (CoA) decarboxylase, based on crystallographic snapshots along the, catalytic cycle and kinetic data on active site mutants. First, we provide, crystallographic evidence that, upon binding of oxalyl-CoA, the C-terminal, 13 residues fold over the substrate, aligning the substrate alpha-carbon, for attack by the ThDP-C2 atom. The second structure presented shows a, covalent reaction intermediate after decarboxylation, interpreted as being, nonplanar. Finally, the structure of a product complex is ... [(full description)]
About this StructureAbout this Structure
2JI8 is a [Single protein] structure of sequence from [Oxalobacter formigenes] with MG, TPP, ADP, FYN and PGE as [ligands]. Active as [[1]], with EC number [4.1.1.8]. Full crystallographic information is available from [OCA].
ReferenceReference
Crystallographic snapshots of oxalyl-CoA decarboxylase give insights into catalysis by nonoxidative ThDP-dependent decarboxylases., Berthold CL, Toyota CG, Moussatche P, Wood MD, Leeper F, Richards NG, Lindqvist Y, Structure. 2007 Jul;15(7):853-61. PMID:17637344
Page seeded by OCA on Mon Oct 29 18:24:24 2007
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Oxalobacter formigenes
- Single protein
- Berthold, C.L.
- Leeper, F.
- Lindqvist, Y.
- Moussatche, P.
- Richards, N.G.J.
- Toyota, C.G.
- Wood, M.D.
- ADP
- FYN
- MG
- PGE
- TPP
- Decarboxylase
- Flavoprotein
- Lyase
- Non- oxidative decarboxylase
- Oxalate degradation
- Product complex
- Thiamin diphosphate-dependent
- Thiamine pyrophosphate