1p0r
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Solution Structure of UBL5 a human Ubiquitin-Like Protein
OverviewOverview
UBL5 is a widely expressed human protein that is strongly conserved across, phylogeny. Orthologs of UBL5 occur in every eukaryotic genome, characterized to date. The yeast ortholog of UBL5, HUB1, was reported to, be a ubiquitin-like protein modifier important for modulation of protein, function. However, unlike ubiquitin and all other ubiquitin-like, modifiers, UBL5 and its yeast ortholog HUB1 both contain a C-terminal, di-tyrosine motif followed by a single variable residue instead of the, characteristic di-glycine found in all other ubiquitin-like modifiers., Here we describe the three-dimensional structure of UBL5 determined by, NMR. The overall structure of the protein was found to be very similar to, ubiquitin despite the low approximately 25% residue similarity. The, signature C-terminal di-tyrosine residues in UBL5 are involved in the, final beta sheet of the protein. This is very different to the di-glycine, motif found in ubiquitin, which extends beyond the final beta sheet. In, addition, we have confirmed an earlier report of an interaction between, UBL5 and the cyclin-like kinase, CLK4, which we have determined is, specific and does not extend to other cyclin-like kinase family members.
About this StructureAbout this Structure
1P0R is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structural analysis of UBL5, a novel ubiquitin-like modifier., McNally T, Huang Q, Janis RS, Liu Z, Olejniczak ET, Reilly RM, Protein Sci. 2003 Jul;12(7):1562-6. PMID:12824502
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