3v6t

Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstromCrystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom

Structural highlights 3v6t is a 6 chain structure with sequence from Xanthomonas. The December 2014 RCSB PDB Molecule of the Month feature on TAL Effectors by David Goodsell is 10.2210/rcsb_pdb/mom_2014_12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.85Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed TAL effectors, secreted by phytopathogenic bacteria, recognize host DNA sequences through a central domain of tandem repeats. Each repeat comprises 33 to 35 conserved amino acids and targets a specific base pair using two hypervariable residues (known as RVD) at positions 12 and 13. Here, we report the crystal structures of a 11.5-repeat TAL effector in both DNA-free and DNA-bound states. Each TAL repeat comprises two helices connected by a short RVD-containing loop. The 11.5 repeats form a right-handed, super-helical structure that tracks along the sense strand of DNA duplex, with RVDs contacting the major groove. The 12th residue stabilizes the RVD loop, whereas the 13th residue makes a base-specific contact. Understanding DNA recognition by TAL effectors may facilitate rational design of DNA-binding proteins with biotechnological applications. Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.,Deng D, Yan C, Pan X, Mahfouz M, Wang J, Zhu JK, Shi Y, Yan N Science. 2012 Jan 5. PMID:22223738 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also TAL effector

spinqualitylabelsall models 3v6t, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image