4le8

Structure of the Als3 adhesin from Candida albicans, residues 1-299 (mature sequence)Structure of the Als3 adhesin from Candida albicans, residues 1-299 (mature sequence)

Structural highlights

4le8 is a 2 chain structure with sequence from Candida albicans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.75Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ALS3_CANAL Cell surface adhesion protein which mediates both yeast-to-host tissue adherence and yeast aggregation. Plays an important role in the biofilm formation and pathogenesis of C.albicans infections. Necessary for C.albicans to bind to N-cadherin on endothelial cells and E-cadherin on oral epithelial cells and subsequent endocytosis by these cells. During disseminated infection, mediates initial trafficking to the brain and renal cortex and contributes to fungal persistence in the kidneys.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8] ^[9] ^[10] ^[11]

References

↑ Sheppard DC, Yeaman MR, Welch WH, Phan QT, Fu Y, Ibrahim AS, Filler SG, Zhang M, Waring AJ, Edwards JE Jr. Functional and structural diversity in the Als protein family of Candida albicans. J Biol Chem. 2004 Jul 16;279(29):30480-9. doi: 10.1074/jbc.M401929200. Epub 2004 , May 5. PMID:15128742 doi:http://dx.doi.org/10.1074/jbc.M401929200
↑ Zhao X, Oh SH, Cheng G, Green CB, Nuessen JA, Yeater K, Leng RP, Brown AJ, Hoyer LL. ALS3 and ALS8 represent a single locus that encodes a Candida albicans adhesin; functional comparisons between Als3p and Als1p. Microbiology. 2004 Jul;150(Pt 7):2415-28. PMID:15256583 doi:http://dx.doi.org/10.1099/mic.0.26943-0
↑ Nobile CJ, Andes DR, Nett JE, Smith FJ, Yue F, Phan QT, Edwards JE, Filler SG, Mitchell AP. Critical role of Bcr1-dependent adhesins in C. albicans biofilm formation in vitro and in vivo. PLoS Pathog. 2006 Jul;2(7):e63. doi: 10.1371/journal.ppat.0020063. PMID:16839200 doi:http://dx.doi.org/10.1371/journal.ppat.0020063
↑ Klotz SA, Gaur NK, De Armond R, Sheppard D, Khardori N, Edwards JE Jr, Lipke PN, El-Azizi M. Candida albicans Als proteins mediate aggregation with bacteria and yeasts. Med Mycol. 2007 Jun;45(4):363-70. doi: 10.1080/13693780701299333. PMID:17510860 doi:http://dx.doi.org/10.1080/13693780701299333
↑ Nobile CJ, Schneider HA, Nett JE, Sheppard DC, Filler SG, Andes DR, Mitchell AP. Complementary adhesin function in C. albicans biofilm formation. Curr Biol. 2008 Jul 22;18(14):1017-24. doi: 10.1016/j.cub.2008.06.034. PMID:18635358 doi:http://dx.doi.org/10.1016/j.cub.2008.06.034
↑ Aoki W, Kitahara N, Miura N, Morisaka H, Kuroda K, Ueda M. Profiling of adhesive properties of the agglutinin-like sequence (ALS) protein family, a virulent attribute of Candida albicans. FEMS Immunol Med Microbiol. 2012 Jun;65(1):121-4. doi:, 10.1111/j.1574-695X.2012.00941.x. Epub 2012 Mar 6. PMID:22321066 doi:http://dx.doi.org/10.1111/j.1574-695X.2012.00941.x
↑ Monroy-Perez E, Sainz-Espunes T, Paniagua-Contreras G, Negrete-Abascal E, Rodriguez-Moctezuma JR, Vaca S. Frequency and expression of ALS and HWP1 genotypes in Candida albicans strains isolated from Mexican patients suffering from vaginal candidosis. Mycoses. 2012 May;55(3):e151-7. doi: 10.1111/j.1439-0507.2012.02188.x. Epub 2012 , Mar 19. PMID:22429754 doi:http://dx.doi.org/10.1111/j.1439-0507.2012.02188.x
↑ Fanning S, Xu W, Solis N, Woolford CA, Filler SG, Mitchell AP. Divergent targets of Candida albicans biofilm regulator Bcr1 in vitro and in vivo. Eukaryot Cell. 2012 Jul;11(7):896-904. doi: 10.1128/EC.00103-12. Epub 2012 Apr, 27. PMID:22544909 doi:http://dx.doi.org/10.1128/EC.00103-12
↑ Fu Y, Phan QT, Luo G, Solis NV, Liu Y, Cormack BP, Edwards JE Jr, Ibrahim AS, Filler SG. Investigation of the function of Candida albicans Als3 by heterologous expression in Candida glabrata. Infect Immun. 2013 Jul;81(7):2528-35. doi: 10.1128/IAI.00013-13. Epub 2013 Apr, 29. PMID:23630968 doi:http://dx.doi.org/10.1128/IAI.00013-13
↑ Alsteens D, Van Dijck P, Lipke PN, Dufrene YF. Quantifying the forces driving cell-cell adhesion in a fungal pathogen. Langmuir. 2013 Nov 5;29(44):13473-80. doi: 10.1021/la403237f. Epub 2013 Oct 23. PMID:24152214 doi:http://dx.doi.org/10.1021/la403237f
↑ Dutton LC, Nobbs AH, Jepson K, Jepson MA, Vickerman MM, Aqeel Alawfi S, Munro CA, Lamont RJ, Jenkinson HF. O-mannosylation in Candida albicans enables development of interkingdom biofilm communities. mBio. 2014 Apr 15;5(2):e00911. doi: 10.1128/mBio.00911-14. PMID:24736223 doi:http://dx.doi.org/10.1128/mBio.00911-14

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OCA

[1] Sheppard DC, Yeaman MR, Welch WH, Phan QT, Fu Y, Ibrahim AS, Filler SG, Zhang M, Waring AJ, Edwards JE Jr. Functional and structural diversity in the Als protein family of Candida albicans. J Biol Chem. 2004 Jul 16;279(29):30480-9. doi: 10.1074/jbc.M401929200. Epub 2004 , May 5. PMID:15128742 doi:http://dx.doi.org/10.1074/jbc.M401929200

[2] Zhao X, Oh SH, Cheng G, Green CB, Nuessen JA, Yeater K, Leng RP, Brown AJ, Hoyer LL. ALS3 and ALS8 represent a single locus that encodes a Candida albicans adhesin; functional comparisons between Als3p and Als1p. Microbiology. 2004 Jul;150(Pt 7):2415-28. PMID:15256583 doi:http://dx.doi.org/10.1099/mic.0.26943-0

[3] Nobile CJ, Andes DR, Nett JE, Smith FJ, Yue F, Phan QT, Edwards JE, Filler SG, Mitchell AP. Critical role of Bcr1-dependent adhesins in C. albicans biofilm formation in vitro and in vivo. PLoS Pathog. 2006 Jul;2(7):e63. doi: 10.1371/journal.ppat.0020063. PMID:16839200 doi:http://dx.doi.org/10.1371/journal.ppat.0020063

[4] Klotz SA, Gaur NK, De Armond R, Sheppard D, Khardori N, Edwards JE Jr, Lipke PN, El-Azizi M. Candida albicans Als proteins mediate aggregation with bacteria and yeasts. Med Mycol. 2007 Jun;45(4):363-70. doi: 10.1080/13693780701299333. PMID:17510860 doi:http://dx.doi.org/10.1080/13693780701299333

[5] Nobile CJ, Schneider HA, Nett JE, Sheppard DC, Filler SG, Andes DR, Mitchell AP. Complementary adhesin function in C. albicans biofilm formation. Curr Biol. 2008 Jul 22;18(14):1017-24. doi: 10.1016/j.cub.2008.06.034. PMID:18635358 doi:http://dx.doi.org/10.1016/j.cub.2008.06.034

[6] Aoki W, Kitahara N, Miura N, Morisaka H, Kuroda K, Ueda M. Profiling of adhesive properties of the agglutinin-like sequence (ALS) protein family, a virulent attribute of Candida albicans. FEMS Immunol Med Microbiol. 2012 Jun;65(1):121-4. doi:, 10.1111/j.1574-695X.2012.00941.x. Epub 2012 Mar 6. PMID:22321066 doi:http://dx.doi.org/10.1111/j.1574-695X.2012.00941.x

[7] Monroy-Perez E, Sainz-Espunes T, Paniagua-Contreras G, Negrete-Abascal E, Rodriguez-Moctezuma JR, Vaca S. Frequency and expression of ALS and HWP1 genotypes in Candida albicans strains isolated from Mexican patients suffering from vaginal candidosis. Mycoses. 2012 May;55(3):e151-7. doi: 10.1111/j.1439-0507.2012.02188.x. Epub 2012 , Mar 19. PMID:22429754 doi:http://dx.doi.org/10.1111/j.1439-0507.2012.02188.x

[8] Fanning S, Xu W, Solis N, Woolford CA, Filler SG, Mitchell AP. Divergent targets of Candida albicans biofilm regulator Bcr1 in vitro and in vivo. Eukaryot Cell. 2012 Jul;11(7):896-904. doi: 10.1128/EC.00103-12. Epub 2012 Apr, 27. PMID:22544909 doi:http://dx.doi.org/10.1128/EC.00103-12

[9] Fu Y, Phan QT, Luo G, Solis NV, Liu Y, Cormack BP, Edwards JE Jr, Ibrahim AS, Filler SG. Investigation of the function of Candida albicans Als3 by heterologous expression in Candida glabrata. Infect Immun. 2013 Jul;81(7):2528-35. doi: 10.1128/IAI.00013-13. Epub 2013 Apr, 29. PMID:23630968 doi:http://dx.doi.org/10.1128/IAI.00013-13

[10] Alsteens D, Van Dijck P, Lipke PN, Dufrene YF. Quantifying the forces driving cell-cell adhesion in a fungal pathogen. Langmuir. 2013 Nov 5;29(44):13473-80. doi: 10.1021/la403237f. Epub 2013 Oct 23. PMID:24152214 doi:http://dx.doi.org/10.1021/la403237f

[11] Dutton LC, Nobbs AH, Jepson K, Jepson MA, Vickerman MM, Aqeel Alawfi S, Munro CA, Lamont RJ, Jenkinson HF. O-mannosylation in Candida albicans enables development of interkingdom biofilm communities. mBio. 2014 Apr 15;5(2):e00911. doi: 10.1128/mBio.00911-14. PMID:24736223 doi:http://dx.doi.org/10.1128/mBio.00911-14

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