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Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1)Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1)
Structural highlights
FunctionPRC1_HUMAN Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle.[1] [2] [3] [4] [5] [6] [7] Publication Abstract from PubMedError-free cell division depends on the assembly of the spindle midzone, a specialized array of overlapping microtubules that emerges between segregating chromosomes during anaphase. The molecular mechanisms by which a subset of dynamic microtubules from the metaphase spindle are selected and organized into a stable midzone array are poorly understood. Here, we show using in vitro reconstitution assays that PRC1 and kinesin-4, two microtubule-associated proteins required for midzone assembly, can tag microtubule plus ends. Remarkably, the size of these tags is proportional to filament length. We determine the crystal structure of the PRC1 homodimer and map the protein-protein interactions needed for tagging microtubule ends. Importantly, length-dependent microtubule plus-end-tagging by PRC1 is also observed in dividing cells. Our findings suggest how biochemically similar microtubules can be differentially marked, based on length, for selective regulation during the formation of specialized arrays, such as those required for cytokinesis. Marking and Measuring Single Microtubules by PRC1 and Kinesin-4.,Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM Cell. 2013 Jul 18;154(2):377-90. doi: 10.1016/j.cell.2013.06.021. PMID:23870126[8] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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