1mc0
Regulatory Segment of Mouse 3',5'-Cyclic Nucleotide Phosphodiesterase 2A, Containing the GAF A and GAF B Domains
OverviewOverview
Cyclic nucleotide phosphodiesterases (PDEs) regulate all pathways that use cGMP or cAMP as a second messenger. Five of the 11 PDE families have regulatory segments containing GAF domains, 3 of which are known to bind cGMP. In PDE2 binding of cGMP to the GAF domain causes an activation of the catalytic activity by a mechanism that apparently is shared even in the adenylyl cyclase of Anabaena, an organism separated from mouse by 2 billion years of evolution. The 2.9-A crystal structure of the mouse PDE2A regulatory segment reported in this paper reveals that the GAF A domain functions as a dimerization locus. The GAF B domain shows a deeply buried cGMP displaying a new cGMP-binding motif and is the first atomic structure of a physiological cGMP receptor with bound cGMP. Moreover, this cGMP site is located well away from the region predicted by previous mutagenesis and structural genomic approaches.
About this StructureAbout this Structure
1MC0 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
The two GAF domains in phosphodiesterase 2A have distinct roles in dimerization and in cGMP binding., Martinez SE, Wu AY, Glavas NA, Tang XB, Turley S, Hol WG, Beavo JA, Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13260-5. Epub 2002 Sep 23. PMID:12271124 Page seeded by OCA on Sat May 3 00:52:25 2008