5gsl

Glycoside hydrolase AGlycoside hydrolase A

Structural highlights

5gsl is a 2 chain structure with sequence from Pyrococcus horikoshii OT3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLMA_PYRHO Exo-type enzyme that specifically cleaves the non-reducing terminal glycosidic bond of chitooligosaccharides (PubMed:16912928, PubMed:28130448). Catalyzes the hydrolysis of GlcN-GlcNAc to glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) (PubMed:28130448). Involved in chitin degradation (PubMed:16912928). Can also hydrolyze chitosan and chitooligosaccharides of various chain lengths (PubMed:16912928).^[1] ^[2]

References

↑ Liu B, Li Z, Hong Y, Ni J, Sheng D, Shen Y. Cloning, expression and characterization of a thermostable exo-beta-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus horikoshii. Biotechnol Lett. 2006 Oct;28(20):1655-60. PMID:16912928 doi:10.1007/s10529-006-9137-0
↑ Mine S, Watanabe M, Kamachi S, Abe Y, Ueda T. The Structure of an Archaeal β-Glucosaminidase Provides Insight into Glycoside Hydrolase Evolution. J Biol Chem. 2017 Mar 24;292(12):4996-5006. PMID:28130448 doi:10.1074/jbc.M116.766535

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OCA

[1] Liu B, Li Z, Hong Y, Ni J, Sheng D, Shen Y. Cloning, expression and characterization of a thermostable exo-beta-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus horikoshii. Biotechnol Lett. 2006 Oct;28(20):1655-60. PMID:16912928 doi:10.1007/s10529-006-9137-0

[2] Mine S, Watanabe M, Kamachi S, Abe Y, Ueda T. The Structure of an Archaeal β-Glucosaminidase Provides Insight into Glycoside Hydrolase Evolution. J Biol Chem. 2017 Mar 24;292(12):4996-5006. PMID:28130448 doi:10.1074/jbc.M116.766535

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