1l2h
Crystal structure of Interleukin 1-beta F42W/W120F mutant
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OverviewOverview
The crystal structure at 1.54 A resolution of a double mutant of, interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was, determined by multiple-wavelength anomalous dispersion (MAD) using data, from highly twinned selenomethionine-modified crystals. The space group is, P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation, function analysis and various intensity statistics revealed the presence, of merohedral twinning in crystals of both the native (twinning fraction, alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms., Structure determination and refinement are discussed with emphasis on the, possible reasons for successful phasing using untreated twinned MAD data.
DiseaseDisease
Known disease associated with this structure: Gastric cancer risk after H. pylori infection OMIM:[147720]
About this StructureAbout this Structure
1L2H is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Use of multiple anomalous dispersion to phase highly merohedrally twinned crystals of interleukin-1beta., Rudolph MG, Kelker MS, Schneider TR, Yeates TO, Oseroff V, Heidary DK, Jennings PA, Wilson IA, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):290-8. Epub 2003, Jan 23. PMID:12554939
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