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Structure of HEWL using Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic PhaseStructure of HEWL using Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase
Structural highlights
FunctionLYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedSerial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP-SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP-SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein. Serial femtosecond crystallography of soluble proteins in lipidic cubic phase.,Fromme R, Ishchenko A, Metz M, Chowdhury SR, Basu S, Boutet S, Fromme P, White TA, Barty A, Spence JC, Weierstall U, Liu W, Cherezov V IUCrJ. 2015 Aug 4;2(Pt 5):545-51. doi: 10.1107/S2052252515013160. eCollection, 2015 Sep 1. PMID:26306196[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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