1j7o
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Solution structure of Calcium-calmodulin N-terminal domain
OverviewOverview
The solution structure of Ca(2+)-ligated calmodulin is determined from, residual dipolar couplings measured in a liquid crystalline medium and, from a large number of heteronuclear J couplings for defining side chains., Although the C-terminal domain solution structure is similar to the X-ray, crystal structure, the EF hands of the N-terminal domain are considerably, less open. The substantial differences in interhelical angles correspond, to negligible changes in short interproton distances and, therefore, cannot be identified by comparison of NOEs and X-ray data. NOE analysis, however, excludes a two-state equilibrium in which the closed apo, conformation is partially populated in the Ca(2+)-ligated state. The, difference between the crystal and solution structures of, Ca(2+)-calmodulin indicates considerable backbone plasticity within the, domains of calmodulin, which is key to their ability to bind a wide range, of targets. In contrast, the vast majority of side chains making up the, target binding surface are locked into the same chi(1) rotameric states as, in complexes with target peptide.
DiseaseDisease
Known diseases associated with this structure: Cavernous malformations of CNS and retina OMIM:[604214], Cerebral cavernous malformations-1 OMIM:[604214], Hyperkeratotic cutaneous capillary-venous malformations associated with cerebral capillary malformations OMIM:[604214], Leukemia, acute T-cell lymphoblastic OMIM:[603025], Leukemia, acute myeloid OMIM:[603025]
About this StructureAbout this Structure
1J7O is a Single protein structure of sequence from Homo sapiens with CA as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of Ca(2+)-calmodulin reveals flexible hand-like properties of its domains., Chou JJ, Li S, Klee CB, Bax A, Nat Struct Biol. 2001 Nov;8(11):990-7. PMID:11685248
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