1j5k
COMPLEX OF THE KH3 DOMAIN OF HNRNP K WITH A SINGLE_STRANDED 10MER DNA OLIGONUCLEOTIDE
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OverviewOverview
To elucidate the basis of sequence-specific single-stranded (ss) DNA, recognition by K homology (KH) domains, we have solved the solution, structure of a complex between the KH3 domain of the transcriptional, regulator heterogeneous nuclear ribonucleoprotein K (hnRNP K) and a 10mer, ssDNA. We show that hnRNP K KH3 specifically recognizes a tetrad of, sequence 5'd-TCCC. The complex is stabilized by a dense network of, methyl-oxygen hydrogen bonds involving the methyl groups of three, isoleucine residues and the O2 and N3 atoms of the two central cytosine, bases. Comparison with the recently solved structure of a specific, protein-ssDNA complex involving the KH3 and KH4 domains of the far, upstream element (FUSE) binding protein FBP suggests that the amino acid, located five residues N-terminal of the invariant GXXG motif, which is, characteristic of all KH domains, plays a crucial role in discrimination, of the first two bases of the tetrad.
About this StructureAbout this Structure
1J5K is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Molecular basis of sequence-specific single-stranded DNA recognition by KH domains: solution structure of a complex between hnRNP K KH3 and single-stranded DNA., Braddock DT, Baber JL, Levens D, Clore GM, EMBO J. 2002 Jul 1;21(13):3476-85. PMID:12093748
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