2jff
CRYSTAL STRUCTURE OF MURD LIGASE IN COMPLEX WITH D-GLU CONTAINING SULFONAMIDE INHIBITOR
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OverviewOverview
Mur ligases play an essential role in the intracellular biosynthesis of, bacterial peptidoglycan, the main component of the bacterial cell wall, and represent attractive targets for the design of novel antibacterials., UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) catalyses the, addition of D-glutamic acid to the cytoplasmic intermediate, UDP-N-acetylmuramoyl-L-alanine (UMA) and is the second in the series of, Mur ligases. MurD ligase is highly stereospecific for its substrate, D-glutamic acid (D-Glu). Here, we report the high resolution crystal, structures of MurD in complexes with two novel inhibitors designed to, mimic the transition state of the reaction, which contain either the D-Glu, or the L-Glu moiety. The binding modes of N-sulfonyl-D-Glu and, N-sulfonyl-L-Glu ... [(full description)]
About this StructureAbout this Structure
2JFF is a [Single protein] structure of sequence from [Escherichia coli] with SO4 and LK2 as [ligands]. Active as [[1]], with EC number [6.3.2.9]. Full crystallographic information is available from [OCA].
ReferenceReference
Structural and functional characterization of enantiomeric glutamic acid derivatives as potential transition state analogue inhibitors of MurD ligase., Kotnik M, Humljan J, Contreras-Martel C, Oblak M, Kristan K, Herve M, Blanot D, Urleb U, Gobec S, Dessen A, Solmajer T, J Mol Biol. 2007 Jun 29;370(1):107-15. Epub 2007 May 4. PMID:17507028
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