4v6o

Structural characterization of mRNA-tRNA translocation intermediates (class 4a of the six classes)Structural characterization of mRNA-tRNA translocation intermediates (class 4a of the six classes)

4v6o, resolution 14.70Å Show:Asymmetric Unit Biological Assembly Structural highlights 4v6o is a 10 chain structure with sequence from Escherichia coli and Sulfolobus acidocaldarius. This structure supersedes the now removed PDB entries 3j0v and 3j0w. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS3_ECOLI Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation (By similarity). Plays a role in mRNA unwinding by the ribosome, possibly by forming part of a processivity clamp. Publication Abstract from PubMed Cryo-EM analysis of a wild-type Escherichia coli pretranslocational sample has revealed the presence of previously unseen intermediate substates of the bacterial ribosome during the first phase of translocation, characterized by intermediate intersubunit rotations, L1 stalk positions, and tRNA configurations. Furthermore, we describe the domain rearrangements in quantitative terms, which has allowed us to characterize the processivity and coordination of the conformational reorganization of the ribosome, along with the associated changes in tRNA ribosome-binding configuration. The results are consistent with the view of the ribosome as a molecular machine employing Brownian motion to reach a functionally productive state via a series of substates with incremental changes in conformation. Structural characterization of mRNA-tRNA translocation intermediates.,Agirrezabala X, Liao HY, Schreiner E, Fu J, Ortiz-Meoz RF, Schulten K, Green R, Frank J Proc Natl Acad Sci U S A. 2012 Mar 30. PMID:22467828 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures Transfer RNA (tRNA)