Chorismate synthase

Function Chorismate synthase (CS) or 5-enolpyruvylshikimate-3-phosphate phopholyase is the seventh enzyme in the shikimate pathway which catalyzes the transition of phosphenol pyruvate to chorismate in prokaryotic bacteria and plants. Chorismate is a precursor for the synthesis of aromatic amino acids in the bacteria. Cs also plays a role in the biosynthesis of nucleotides. CS catalyzes the conversion of 5-enolpyruvylshikimate-3-phosphate (EPSP) to chorismate. The activity of CS requires the presence of FMN. Relevance CS inhibitors which inhibit the shikimate pathway, serve as antimicrobial agents. Structural highlights The 3D structure of CS complex with its cofactor FMN and its substrate EPSP shows the EPSP binding site as very hydrophobic containing numerous Arg. Lys and His residues. The FMN cofactor has numerous interactions with CS and its phosphate moiety is located in the dimer interface and interacts with residues of the other monomer as well.

spinqualitylabelsall models Se-Met structure of chorismate synthase complex with FMN, EPSP substrate, cobalt hexaamine (blue) and ethylene glycol (PDB code 1qxo) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D structures of chorismate synthase3D structures of chorismate synthase

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• Chorismate synthase
  • 1rs2, 1r52, 1r53 - CS - yeast
    
  • 4ob9, 4lj2, 4o90. 5wuy - CS - Acinetobacter baumannii
    
  • 4ecd - CS - Bifidobacterium longum
    
  • 1sq1 - CS - Campylobacter jejuni
    
  • 2g85, 2o11, 1ztb, 4bai, 4baj - MtCS - Mycobacterium tuberculosis
    
  • 1umf - HpCS - Helicobacter pylori
    
  • 5z9a - CS - Pseudomonas aeruginosa
    
  • 1q1l - CS - Aquifex aeolicus
    
  • 7rca - CS - Candida lusitaniae
    
• Chorismate synthase complex
  • 1um0 - HpCS + FMN
    
  • 1qxo - CS + FMN + 5-enolpyruvylshikimate-3-phosphate - Streprococcus pneumoniae
    
  • 2qhf - MtCS + nicotinamide