1ik1
Solution Structure of an RNA Hairpin from HRV-14
OverviewOverview
The 5' noncoding region of the picornaviral genome begins with a cloverleaf which is required for viral replication, due at least in part to an interaction with the viral RNA polymerase as part of a fusion with the predominant viral protease. The necessary region of the cloverleaf has previously been narrowed to a highly conserved stem-loop. The solution structure of a 14-nucleotide RNA hairpin, which is part of the conserved stem-loop from human rhinovirus isotype 14, is presented here. The secondary structure of the hairpin is identical to predictions: a five base pair stem is bounded by a triloop with sequence UAU. However, the fold of the triloop is novel, with stacking of the second loop base onto the closing base pair of the stem, and deviations from A form geometry are introduced into the stem regions bordering the triloop, particularly on the 3' side. These deviations and the associated triloop structure could help to explain the distinct sequence conservation and mutational analysis data observed for the stem region of the hairpin, as compared to a second sequentially similar stem in the intact stem-loop.
About this StructureAbout this Structure
1IK1 is a Single protein structure. Full crystallographic information is available from OCA.
ReferenceReference
Structure of an RNA hairpin from HRV-14., Huang H, Alexandrov A, Chen X, Barnes TW 3rd, Zhang H, Dutta K, Pascal SM, Biochemistry. 2001 Jul 10;40(27):8055-64. PMID:11434774 Page seeded by OCA on Fri May 2 20:05:14 2008