1iaw
CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA
OverviewOverview
NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase activities when a Lys residue is substituted for Leu 43. The NaeI-DNA structure demonstrates that each of the two domains of NaeI recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 A to grip DNA, widening that of the Endo domain 8 A to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA structure presents the first example of novel recognition of two copies of one DNA sequence by two different amino acid sequences and two different structural motifs in one polypeptide.
About this StructureAbout this Structure
1IAW is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Full crystallographic information is available from OCA.
ReferenceReference
Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement., Huai Q, Colandene JD, Topal MD, Ke H, Nat Struct Biol. 2001 Aug;8(8):665-9. PMID:11473254 Page seeded by OCA on Fri May 2 19:47:08 2008