1ggl
HUMAN CELLULAR RETINOL BINDING PROTEIN III
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OverviewOverview
Two cellular retinol-binding proteins (CRBP I and II) with distinct tissue, distributions and retinoid-binding properties have been recognized thus, far in mammals. Here, we report the identification of a human, retinol-binding protein resembling type I (55.6% identity) and type II, (49.6% identity) CRBPs, but with a unique H residue in the, retinoid-binding site and a distinctively different tissue distribution., Additionally, this binding protein (CRBP III) exhibits a remarkable, sequence identity (62.2%) with the recently identified, iota-crystallin/CRBP of the diurnal gecko Lygodactylus picturatus [Werten, P. J. L., Roll, B., van Alten, D. M. F. & de Jong, W. W. (2000) Proc., Natl. Acad. Sci. USA 97, 3282-3287 (First Published March 21, 2000;, 10.1073/pnas.050500597)]. CRBP III and all-trans-retinol form a complex, (K(d) approximately 60 nM), the absorption spectrum of which is, characterized by the peculiar fine structure typical of the spectra of, holo-CRBP I and II. As revealed by a 2.3-A x-ray molecular model of, apo-CRBP III, the amino acid residues that line the retinol-binding site, in CRBP I and II are positioned nearly identically in the structure of, CRBP III. At variance with the human CRBP I and II mRNAs, which are most, abundant in ovary and intestine, respectively, the CRBP III mRNA is, expressed at the highest levels in kidney and liver thus suggesting a, prominent role for human CRBP III as an intracellular mediator of retinol, metabolism in these tissues.
DiseaseDisease
Known disease associated with this structure: Chondrosarcoma, extraskeletal myxoid OMIM:[601574]
About this StructureAbout this Structure
1GGL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Identification, retinoid binding, and x-ray analysis of a human retinol-binding protein., Folli C, Calderone V, Ottonello S, Bolchi A, Zanotti G, Stoppini M, Berni R, Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3710-5. PMID:11274389
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