3iz4

Modified E. coli tmRNA in the resume state with the tRNA-like domain in the ribosomal P site interacting with the SmpBModified E. coli tmRNA in the resume state with the tRNA-like domain in the ribosomal P site interacting with the SmpB

3iz4, resolution 13.60Å Show:Asymmetric Unit Biological Assembly Structural highlights 3iz4 is a 2 chain structure with sequence from [1] and "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. The January 2013 RCSB PDB Molecule of the Month feature on Transfer-Messenger RNA by David Goodsell is 10.2210/rcsb_pdb/mom_2013_1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [SSRP_THET8] Binds specifically to the SsrA RNA (tmRNA) and is required for stable association of SsrA with ribosomes (By similarity). Publication Abstract from PubMed Bacterial ribosomes stalled by truncated mRNAs are rescued by transfer-messenger RNA (tmRNA), a dual-function molecule that contains a tRNA-like domain (TLD) and an internal open reading frame (ORF). Occupying the empty A site with its TLD, the tmRNA enters the ribosome with the help of elongation factor Tu and a protein factor called small protein B (SmpB), and switches the translation to its own ORF. In this study, using cryo-electron microscopy, we obtained the first structure of an in vivo-formed complex containing ribosome and the tmRNA at the point where the TLD is accommodated into the ribosomal P site. We show that tmRNA maintains a stable 'arc and fork' structure on the ribosome when its TLD moves to the ribosomal P site and translation resumes on its ORF. Based on the density map, we built an atomic model, which suggests that SmpB interacts with the five nucleotides immediately upstream of the resume codon, thereby determining the correct selection of the reading frame on the ORF of tmRNA. Visualizing the transfer-messenger RNA as the ribosome resumes translation.,Fu J, Hashem Y, Wower I, Lei J, Liao HY, Zwieb C, Wower J, Frank J EMBO J. 2010 Nov 17;29(22):3819-25. Epub 2010 Oct 12. PMID:20940705 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.