1c9q
AVERAGE NMR SOLUTION STRUCTURE OF THE BIR-2 DOMAIN OF XIAP
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OverviewOverview
The inhibitor-of-apoptosis (IAP) family of proteins, originally identified, in baculoviruses, regulate programmed cell death in a variety of, organisms. IAPs inhibit specific enzymes (caspases) in the death cascade, and contain one to three modules of a common 70-amino-acid motif called, the BIR domain. Here we describe the nuclear magnetic resonance structure, of a region encompassing the second BIR domain (BIR2) of a human IAP, family member, XIAP (also called hILP or MIHA). The structure of the BIR, domain consists of a three-stranded antiparallel beta-sheet and four, alpha-helices and resembles a classical zinc finger. Unexpectedly, conserved amino acids within the linker region between the BIR1 and BIR2, domains were found to be critical for inhibiting caspase-3. The absence or, presence of these residues may explain the differences in caspase, inhibition observed for different truncated and full-length IAPs. Our data, further indicate that these residues may bind to the active site and that, the BIR domain may interact with an adjacent site on the enzyme.
DiseaseDisease
Known diseases associated with this structure: Lymphoproliferative syndrome, X-linked, 2 OMIM:[300079]
About this StructureAbout this Structure
1C9Q is a Single protein structure of sequence from Homo sapiens with ZN as ligand. Full crystallographic information is available from OCA.
ReferenceReference
NMR structure and mutagenesis of the inhibitor-of-apoptosis protein XIAP., Sun C, Cai M, Gunasekera AH, Meadows RP, Wang H, Chen J, Zhang H, Wu W, Xu N, Ng SC, Fesik SW, Nature. 1999 Oct 21;401(6755):818-22. PMID:10548111
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