7kh5
Hen Egg White Lysozyme in complex with tetrabromoterephthalic acidHen Egg White Lysozyme in complex with tetrabromoterephthalic acid
Structural highlights
Function[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedThe phase problem is a persistent bottleneck that impedes the structure-determination pipeline and must be solved to obtain atomic resolution crystal structures of macromolecules. Although molecular replacement has become the predominant method of solving the phase problem, many scenarios still exist in which experimental phasing is needed. Here, a proof-of-concept study is presented that shows the efficacy of using tetrabromoterephthalic acid (B4C) as an experimental phasing compound. Incorporating B4C into the crystal lattice using co-crystallization, the crystal structure of hen egg-white lysozyme was solved using MAD phasing. The strong anomalous signal generated by its four Br atoms coupled with its compatibility with commonly used crystallization reagents render B4C an effective experimental phasing compound that can be used to overcome the phase problem. Simplified heavy-atom derivatization of protein structures via co-crystallization with the MAD tetragon tetrabromoterephthalic acid.,Truong JQ, Nguyen S, Bruning JB, Shearwin KE Acta Crystallogr F Struct Biol Commun. 2021 May 1;77(Pt 5):156-162. doi:, 10.1107/S2053230X21004052. Epub 2021 Apr 28. PMID:33949976[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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