2iuw
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CRYSTAL STRUCTURE OF HUMAN ABH3 IN COMPLEX WITH IRON ION AND 2-OXOGLUTARATE
OverviewOverview
Methylating agents are ubiquitous in the environment, and central in, cancer therapy. The 1-methyladenine and 3-methylcytosine lesions in, DNA/RNA contribute to the cytotoxicity of such agents. These lesions are, directly reversed by ABH3 (hABH3) in humans and AlkB in Escherichia coli., Here, we report the structure of the hABH3 catalytic core in complex with, iron and 2-oxoglutarate (2OG) at 1.5 A resolution and analyse key, site-directed mutants. The hABH3 structure reveals the beta-strand, jelly-roll fold that coordinates a catalytically active iron centre by a, conserved His1-X-Asp/Glu-X(n)-His2 motif. This experimentally establishes, hABH3 as a structural member of the Fe(II)/2OG-dependent dioxygenase, superfamily, which couples substrate oxidation to conversion of 2OG into, ... [(full description)]
About this StructureAbout this Structure
2IUW is a [Single protein] structure of sequence from [Homo sapiens] with FE, AKG and BME as [ligands]. Full crystallographic information is available from [OCA].
ReferenceReference
Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage., Sundheim O, Vagbo CB, Bjoras M, Sousa MM, Talstad V, Aas PA, Drablos F, Krokan HE, Tainer JA, Slupphaug G, EMBO J. 2006 Jul 26;25(14):3389-97. Epub 2006 Jul 6. PMID:16858410
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