1adx
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FIFTH EGF-LIKE DOMAIN OF THROMBOMODULIN (TMEGF5), NMR, 14 STRUCTURES
OverviewOverview
The structure of the fifth EGF-like domain (residues Q387 to E426) of, thrombomodulin (TMEGF5) has been determined by two-dimensional NMR. TMEGF5, binds to thrombin with a Ki of 1.9 microM and has been shown to have a, novel disulfide bonding pattern in a fully active fragment of TM. In EGF, the disulfide bonding pattern is (1-3,2-4, 5-6), while TMEGF5 has an, uncrossed (1-2,3-4,5-6) pattern. The structure of this novel domain, determined from 483 NOE-derived distance restraints, appears to have, diverged from the common EGF-like structure. Superposition of the 14, lowest-energy structures of TMEGF5 gives an overall r.m.s.d. of 1.09 A for, the backbone atoms. The central two-stranded beta-sheet common to all, EGF-like domains is not present in TMEGF5. The A loop, residues C390 to, C395, is twisted away from interacting with the B loop, residues C399 to, C407, as in EGF, and is close to the C loop, residues C409 to C421. This, twist causes the N and C termini to be closer together in TMEGF5 than in, EGF. Most of the residues that are important for activity lie on one face, of the molecule, which is likely to be the thrombin-binding surface of the, domain. The structure of the C loop within the domain, which is a, beta-hairpin similar to EGF, is similar to the structure of a synthetic, version of the loop bound to thrombin as determined by transferred NOE, experiments. Despite the similarity in the structures of the loops, the, residues immediately following C421 are in different positions in the two, structures suggesting that these "tail" residues may change conformation, upon thrombin binding.Copyright 1997 Academic Press Limited
DiseaseDisease
Known diseases associated with this structure: Myocardial infarction, susceptibility to OMIM:[188040], Thrombophilia due to thrombomodulin defect OMIM:[188040]
About this StructureAbout this Structure
1ADX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the fifth EGF-like domain of thrombomodulin: An EGF-like domain with a novel disulfide-bonding pattern., Sampoli Benitez BA, Hunter MJ, Meininger DP, Komives EA, J Mol Biol. 1997 Nov 7;273(4):913-26. PMID:9367781
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