1e4p
STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE
OverviewOverview
The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site., Michiels PJ, Schouten CH, Hilbers CW, Heus HA, RNA. 2000 Dec;6(12):1821-32. PMID:11142381 Page seeded by OCA on Fri May 2 14:39:32 2008