Crystal structure of a multi-domain immunophilin from Arabidopsis thalianaCrystal structure of a multi-domain immunophilin from Arabidopsis thaliana
Structural highlights
2if4 is a 1 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
[FKB42_ARATH] PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity). Modulates the uptake of MRP substrates into the vacuole; reduces metolachlor-GS (MOC-GS) and enhances 17-beta-estradiol 17-(beta-D-glucuronide) (E(2)17betaG) uptake. Regulates cell elongation and orientation. Functions as a positive regulator of PGP1-mediated auxin transport. Confers drug modulation of PGP1 efflux activity as interaction with NPA or flavonol quercetin prevents its physical and functional interaction with PGP1. Required for the proper localization of auxin-related ABCB transporters. Plays a role in brassinosteroid (BR) signaling pathway.[1][2][3][4][5][6]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
FKBP42 is a membrane-anchored immunophilin playing a critical role in morphogenesis and development of higher plants. We present the X-ray structure of the cytoplasmic portion of FKBP42 comprising both the FKBP-like domain and the TPR domain at 2.85 A resolution. The data shed light on the probable binding modes of key interaction partners, including HSP90 and two classes of ABC transporters. The resulting models provide a structural background for further investigation of the unique biological properties of this protein.
Crystal structure of a multi-domain immunophilin from Arabidopsis thaliana: a paradigm for regulation of plant ABC transporters.,Granzin J, Eckhoff A, Weiergraber OH J Mol Biol. 2006 Dec 8;364(4):799-809. Epub 2006 Sep 26. PMID:17045295[7]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑Geisler M, Kolukisaoglu HU, Bouchard R, Billion K, Berger J, Saal B, Frangne N, Koncz-Kalman Z, Koncz C, Dudler R, Blakeslee JJ, Murphy AS, Martinoia E, Schulz B. TWISTED DWARF1, a unique plasma membrane-anchored immunophilin-like protein, interacts with Arabidopsis multidrug resistance-like transporters AtPGP1 and AtPGP19. Mol Biol Cell. 2003 Oct;14(10):4238-49. Epub 2003 Aug 7. PMID:14517332 doi:http://dx.doi.org/10.1091/mbc.E02-10-0698
↑Geisler M, Girin M, Brandt S, Vincenzetti V, Plaza S, Paris N, Kobae Y, Maeshima M, Billion K, Kolukisaoglu UH, Schulz B, Martinoia E. Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters. Mol Biol Cell. 2004 Jul;15(7):3393-405. Epub 2004 May 7. PMID:15133126 doi:http://dx.doi.org/10.1091/mbc.E03-11-0831
↑Perez-Perez JM, Ponce MR, Micol JL. The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling. Plant Physiol. 2004 Jan;134(1):101-17. PMID:14730066 doi:http://dx.doi.org/10.1104/pp.103.032524
↑Bouchard R, Bailly A, Blakeslee JJ, Oehring SC, Vincenzetti V, Lee OR, Paponov I, Palme K, Mancuso S, Murphy AS, Schulz B, Geisler M. Immunophilin-like TWISTED DWARF1 modulates auxin efflux activities of Arabidopsis P-glycoproteins. J Biol Chem. 2006 Oct 13;281(41):30603-12. Epub 2006 Aug 3. PMID:16887800 doi:http://dx.doi.org/10.1074/jbc.M604604200
↑Bailly A, Sovero V, Vincenzetti V, Santelia D, Bartnik D, Koenig BW, Mancuso S, Martinoia E, Geisler M. Modulation of P-glycoproteins by auxin transport inhibitors is mediated by interaction with immunophilins. J Biol Chem. 2008 Aug 1;283(31):21817-26. doi: 10.1074/jbc.M709655200. Epub 2008 , May 22. PMID:18499676 doi:http://dx.doi.org/10.1074/jbc.M709655200
↑Wu G, Otegui MS, Spalding EP. The ER-localized TWD1 immunophilin is necessary for localization of multidrug resistance-like proteins required for polar auxin transport in Arabidopsis roots. Plant Cell. 2010 Oct;22(10):3295-304. doi: 10.1105/tpc.110.078360. Epub 2010 Oct , 22. PMID:20971896 doi:http://dx.doi.org/10.1105/tpc.110.078360
↑Granzin J, Eckhoff A, Weiergraber OH. Crystal structure of a multi-domain immunophilin from Arabidopsis thaliana: a paradigm for regulation of plant ABC transporters. J Mol Biol. 2006 Dec 8;364(4):799-809. Epub 2006 Sep 26. PMID:17045295 doi:10.1016/j.jmb.2006.09.052
