2mip
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CRYSTAL STRUCTURE OF HUMAN IMMUNODEFICIENCY VIRUS (HIV) TYPE 2 PROTEASE IN COMPLEX WITH A REDUCED AMIDE INHIBITOR AND COMPARISON WITH HIV-1 PROTEASE STRUCTURES
OverviewOverview
The crystal structure of HIV-2 protease in complex with a reduced amide, inhibitor [BI-LA-398; Phe-Val-Phe-psi (CH2NH)-Leu-Glu-Ile-amide] has been, determined at 2.2-A resolution and refined to a crystallographic R factor, of 17.6%. The rms deviation from ideality in bond lengths is 0.018 A and, in bond angles is 2.8 degrees. The largest structural differences between, HIV-1 and HIV-2 proteases are located at residues 15-20, 34-40, and 65-73, away from the flap region and the substrate binding sites. The rms, distance between equivalent C alpha atoms of HIV-1 and HIV-2 protease, structures excluding these residues is 0.5 A. The shapes of the S1 and S2, pockets in the presence of this inhibitor are essentially unperturbed by, the amino acid differences between HIV-1 and HIV-2 proteases. The, interaction of the inhibitor with HIV-2 protease is similar to that, observed in HIV-1 protease structures. The unprotected N terminus of the, inhibitor interacts with the side chains of Asp-29 and Asp-30. The, glutamate side chain of the inhibitor forms hydrogen bonds with the, main-chain amido groups of residues 129 and 130.
About this StructureAbout this Structure
2MIP is a Single protein structure of sequence from Human immunodeficiency virus 1 with NH2 as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of human immunodeficiency virus (HIV) type 2 protease in complex with a reduced amide inhibitor and comparison with HIV-1 protease structures., Tong L, Pav S, Pargellis C, Do F, Lamarre D, Anderson PC, Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8387-91. PMID:8378311
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