2bz2
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SOLUTION STRUCTURE OF NELF E RRM
OverviewOverview
The elongation of transcription of HIV RNA at the TAR, (transactivation-response element) is highly regulated by positive and, negative factors. The cellular negative transcription elongation factor, NELF (negative elongation factor) was suggested to be involved in, transcriptional regulation of HIV-1 (HIV type 1) by binding to the stem of, the viral TAR RNA which is synthesized by cellular RNA polymerase II at, the viral long terminal repeat. NELF is a heterotetrameric protein, consisting of NELF A, B, C or the splice variant D, and E. In the present, study, we determined the solution structure of the RRM (RNA-recognition, motif) of the RNA-binding subunit NELF E and studied its interaction with, the viral TAR RNA. Our results show that the separately expressed, recombinant NELF E RRM has alpha-helical and beta-strand elements adopting, a betaalphabetabetaalphabeta fold and is able to bind to TAR RNA., Fluorescence equilibrium titrations with fluorescently labelled double-, and single-stranded oligoribonucleotides representing the TAR RNA stem, imply that NELF E RRM binds to the single-stranded TAR RNAs with K(d), values in the low-micromolar range.
About this StructureAbout this Structure
2BZ2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structural studies on the RNA-recognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription., Rao JN, Neumann L, Wenzel S, Schweimer K, Rosch P, Wohrl BM, Biochem J. 2006 Dec 15;400(3):449-56. PMID:16898873
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