Sandbox GGC1

Histone H3.3

This is a default text for your page Sandbox GGC1. Click above on edit this page to modify. Be careful with the < and > signs. You may include any references to papers as in: the use of JSmol in Proteopedia or to the article describing Jmol to the rescue. Function Histone H3 replaces H3 in a range of nucleosomes in active genes and it takes over the original H3 in non dividing cells. Nucleosomes wrap around and compact DNA into chromatin which limits DNA access to cellular machineries which need DNA as a template. Histones play an important role in regulation of transcription, DNA repair, DNA replication and also chromosomal stability. Access to DNA is regulated by post-translational modifications of histones which is called a histone code, and nucleosome remodeling. It also serves as a replacement histone that's imbedded chromatin regions by the HIRA chaperone, after the depletion of the H3.1 during transcription and DNA repair. Disease There have been studies that have identified mutations encoding a K27M substitution and there have also been mutations that encoded GLY 34 to ARG or VAL called the G34R/V substitution. K27M tumors are present in ex: spinal cord, thalamus, pons, brainstem and G34R/V tumors are shown in the cerebral hemispheres. There are mutations in H3.3 that are found in different types of bone tumors like chrondroblastoma for example and giant cell tumors of the bone. Chondroblastomaarises in children and in young adults in the cartilage of the growth plates of the long bones and is most typically benign. Relevance Histone octamer containing two of H2A, H2B, H3 and H4 and the octamer wraps 147bp of DNA. H3.3 interacts with HIRA which is a chaperone and ZMYND11 when trimethylated at HIRA can deposit histones of H3.3 in replicating and non replicating cells. Also in the HIRA complex, Anti slicing factors ASF1a and ASF1b are purified by H3.3. ZMYND11 also known as BS69 can find H3.3 LYS 36 (H3.3K36me3) by the binding domain of chromatin. Structural highlights CENP-Ais a centromere specific variant of Histone H3 and it's controlled in normal cells and its chromosome localization is heavily restricted in the centromere regions. It can be over expressed in cancer cells and also be mislocalized ectopically in the form of heterotypic nucleosomes containing H3.3. In vitro, the human CENP-A nucleosomes can have two copies each of CENP-A, H2A, H2B and H4 histones.

spinqualitylabelsall models Caption for this structure Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

ReferencesReferences

1. Arimura, Y.; Shirayama, K.; Horikoshi, N.; Fujita, R.; Taguchi, H.; Kagawa, W.; Fukagawa, T.; Almouzni, G.; Kurumizaka, H. Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3. https://www.nature.com/articles/srep07115 (accessed Nov 1, 2020).

2. Cancer Discovery Science Writers. Histone H3.3 Mutations Are Cancer Type-Specific. https://cancerdiscovery.aacrjournals.org/content/3/12/1329.1 (accessed Nov 14, 2020).

3. Gianno, F.; Antonelli, M.; Ferretti2018, E.; Massimino, M.; Arcella, A.; Giangaspero, F. Pediatric high-grade glioma: A heterogeneous group of neoplasms with different molecular drivers. (accessed Nov 16, 2020).

4. Kallappagoudar, S.; Yadav, R. K.; Lowe, B. R.; Partridge, J. F. Histone H3 mutations--a special role for H3.3 in tumorigenesis? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446520/ (accessed Nov 1, 2020).

5. Morell, N.; Rajani, R. Chondroblastoma - OrthoInfo - AAOS. https://orthoinfo.aaos.org/en/diseases--conditions/chondroblastoma (accessed Nov 16, 2020).

6.panelRuiGuo111LijuanZheng111Juw WonPark2RuituLv1HaoChen1FangfangJiao1WenqiXu1ShirongMu3HongWen45JinsongQiu6ZhentianWang1PengyuanYang1FeizhenWu1JingyiHui3XiangdongFu6XiaobingShi4512Yujiang GenoShi7812YiXing212…YangShi891012, A. links open overlay; RuiGuo111; 1; 11; LijuanZheng111; Juw WonPark2; 2; RuituLv1; HaoChen1; FangfangJiao1; WenqiXu1; ShirongMu3; 3; HongWen45; 4; 5; JinsongQiu6; 6; ZhentianWang1; PengyuanYang1; FeizhenWu1; JingyiHui3; XiangdongFu6; XiaobingShi4512; 12; Yujiang GenoShi7812; 7; 8; YiXing212; YangShi891012; 9; 10; Highlights•BS69/ZMYND11 binds H3.3K36me3 and colocalizes with H3.3K36me3 in gene bodies•BS69 directly interacts with EFTUD2; SummaryBS69 (also called ZMYND11) contains tandemly arranged PHD. BS69/ZMYND11 Reads and Connects Histone H3.3 Lysine 36 Trimethylation-Decorated Chromatin to Regulated Pre-mRNA Processing. https://reader.elsevier.com/reader/sd/pii/S1097276514006777?token=A4FD3B8CDE2F310EA514C66E96DC4489F79C8EA96F6FC878DCD4BFC066FA809C2E83C8A9B57353A53915171AD2491D4C (accessed Nov 16, 2020).

7. UniProt ConsortiumEuropean Bioinformatics InstituteProtein Information ResourceSIB Swiss Institute of Bioinformatics. Histone H3.3. https://www.uniprot.org/uniprot/P84243 (accessed Nov 1, 2020).

8.Yuen, B. T. K.; Knoepfler, P. S. Histone H3.3 mutations: a variant path to cancer. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3882088/ (accessed Nov 16, 2020).