1htf
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X-RAY CRYSTALLOGRAPHIC STUDIES OF A SERIES OF PENICILLIN-DERIVED ASYMMETRIC INHIBITORS OF HIV-1 PROTEASE
OverviewOverview
In the development of a treatment for AIDS, the HIV-1 protease has been, identified as a good target enzyme for inhibitor design. We previously, reported a series of dimeric penicillin-derived C2-symmetric HIV-1, protease inhibitors [Humber, D., et al. (1993) J. Med. Chem. 36, 3120-3128]. In an attempt to reduce the size and optimize the binding of, these C2-symmetric inhibitors, molecular modeling studies led to a novel, series of monomeric penicillin-derived inhibitors of HIV-1 protease. The, binding modes of these monomeric inhibitors have been characterized by, X-ray crystallographic and NMR studies. Crystal structures of HIV-1, protease complexed to three inhibitors (GR123976, GR126045, and GR137615), from this series identify the molecular details of the interactions. The, binding of GR123976 (IC50 = 2.3 microM) exhibits good hydrophobic contacts, but few electrostatic interactions. A strategy of structure-based design, and chemical synthesis led to the elaboration of GR123976 to optimize, interactions with the protein. Crystallographic analysis of HIV-1 protease, complexed to GR126045 and GR137615 identified these interactions with the, catalytic aspartates and the protein binding pockets. The crystal, structures of the three complexes confirm the presence of the major, interactions modeled in order to optimize potency and reveal details of, the molecular recognition by HIV-1 protease of this novel series of, nonpeptidic inhibitors.
About this StructureAbout this Structure
1HTF is a Single protein structure of sequence from Human immunodeficiency virus 1 with G26 as ligand. Full crystallographic information is available from OCA.
ReferenceReference
X-ray crystallographic studies of a series of penicillin-derived asymmetric inhibitors of HIV-1 protease., Jhoti H, Singh OM, Weir MP, Cooke R, Murray-Rust P, Wonacott A, Biochemistry. 1994 Jul 19;33(28):8417-27. PMID:8031777
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