1hef
THE CRYSTAL STRUCTURES AT 2.2 ANGSTROMS RESOLUTION OF HYDROXYETHYLENE-BASED INHIBITORS BOUND TO HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 PROTEASE SHOW THAT THE INHIBITORS ARE PRESENT IN TWO DISTINCT ORIENTATIONS
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OverviewOverview
As part of a structure-based drug design program directed against enzyme, targets in the human immunodeficiency virus (HIV), we have determined the, three-dimensional structures of the HIV type 1 protease complexed with two, hydroxyethylene-based inhibitors. The inhibitors (SKF 107457 and SKF, 108738) are hexapeptide substrate analogues with the scissile bond being, replaced by a hydroxyethylene isostere. The structures were determined, using x-ray diffraction data to 2.2 A measured at the Cornell High Energy, Synchrotron Source on hexagonal crystals of each of the complexes. The, structures have been extensively refined using a reciprocal space, least-squares method to conventional crystallographic R factors of 0.186, and 0.159, respectively. The protein structure differs from that in the, unliganded state of the enzyme and is most similar to that of the, structure of the other reported (Jaskolski, M., Tomasselli, A. G., Sawyer, T. K., Staples, D. G., Heinrikson, R. L., Schneider, J., Kent, S. B. H., and Wlodawer, A. (1990) Biochemistry 29, 5889-5907) hydroxyethylene-based, inhibitor complex. Unlike in that structure, however, the inhibitors are, observed, in the present crystal structures, in two equally abundant, orientations that are a consequence of the homodimeric nature of the, enzyme coupled with the asymmetric structures of the inhibitors. Although, the differences between the two inhibitors used in the present study are, confined to the P1' site, the van der Waals interactions made by the, inhibitor atoms with the amino acid residues in the protein differ, throughout the structures of the inhibitors.
About this StructureAbout this Structure
1HEF is a Single protein structure of sequence from Human immunodeficiency virus 1 with PHE, OHE and OME as ligands. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structures at 2.2-A resolution of hydroxyethylene-based inhibitors bound to human immunodeficiency virus type 1 protease show that the inhibitors are present in two distinct orientations., Murthy KH, Winborne EL, Minnich MD, Culp JS, Debouck C, J Biol Chem. 1992 Nov 15;267(32):22770-8. PMID:1429626
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