1exq
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CRYSTAL STRUCTURE OF THE HIV-1 INTEGRASE CATALYTIC CORE DOMAIN
OverviewOverview
Insolubility of full-length HIV-1 integrase (IN) limited previous, structure analyses to individual domains. By introducing five point, mutations, we engineered a more soluble IN that allowed us to generate, multidomain HIV-1 IN crystals. The first multidomain HIV-1 IN structure is, reported. It incorporates the catalytic core and C-terminal domains, (residues 52-288). The structure resolved to 2.8 A is a Y-shaped dimer., Within the dimer, the catalytic core domains form the only dimer, interface, and the C-terminal domains are located 55 A apart. A 26-aa, alpha-helix, alpha6, links the C-terminal domain to the catalytic core. A, kink in one of the two alpha6 helices occurs near a known proteolytic, site, suggesting that it may act as a flexible elbow to reorient the, domains during the integration process. Two proteins that bind DNA in a, sequence-independent manner are structurally homologous to the HIV-1 IN, C-terminal domain, suggesting a similar protein-DNA interaction in which, the IN C-terminal domain may serve to bind, bend, and orient viral DNA, during integration. A strip of positively charged amino acids contributed, by both monomers emerges from each active site of the dimer, suggesting a, minimally dimeric platform for binding each viral DNA end. The crystal, structure of the isolated catalytic core domain (residues 52-210), independently determined at 1.6-A resolution, is identical to the core, domain within the two-domain 52-288 structure.
About this StructureAbout this Structure
1EXQ is a Single protein structure of sequence from Human immunodeficiency virus 1 with CD, CL and SO4 as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the HIV-1 integrase catalytic core and C-terminal domains: a model for viral DNA binding., Chen JC, Krucinski J, Miercke LJ, Finer-Moore JS, Tang AH, Leavitt AD, Stroud RM, Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8233-8. PMID:10890912
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