1ceu
NMR STRUCTURE OF THE (1-51) N-TERMINAL DOMAIN OF THE HIV-1 REGULATORY PROTEIN
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OverviewOverview
The human immunodeficiency virus type 1 (HIV-1) genome encodes a highly, conserved 16 kDa regulatory gene product, Vpr (viral protein of, regulation, 96 amino acid residues), which is incorporated into virions, in quantities equivalent to those of the viral Gag proteins. In the, infected cells, Vpr is believed to function in the early phase of HIV-1, replication, including nuclear migration of preintegration complex, transcription of the provirus genome and viral multiplication by blocking, cells in the G2 phase. Vpr has a critical role in long-term AIDS disease, by inducing infection in nondividing cells such as monocytes and, macrophages. Mutations have suggested that the N-terminal domain of Vpr, encompassing the first 40 residues could be required for nuclear, localization, packaging into virions and binding of transcription factor, (TFIIB, Sp1), viral proteins (p6) and cellular proteins (RIP1, UNG, karyopherins). To gain insight into the structure-function relationship of, Vpr, (1-51)Vpr was synthesized and its structure analyzed by circular, dichroism and two-dimensional 1H NMR in aqueous trifluoroethanol (30%), solution and refined by restrained molecular dynamics. The structure is, characterized by three turns around the first three prolines, Pro5, Pro10, Pro14, followed by a long amphipathic alpha helix-turn-alpha helix, (Asp17-Ile46) motif ended by a turn extending from Tyr47 to Thr49. The, alpha helix-turn-alpha helix motif and the amphipathic helix are well, known for being implicated in protein-protein or protein-nucleic acid, interaction. Therefore structural characteristics of the (1-51) N-terminal, fragment of Vpr could explain why this region of Vpr plays a role in, several biological functions of this protein.
About this StructureAbout this Structure
1CEU is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
ReferenceReference
NMR structure of the (1-51) N-terminal domain of the HIV-1 regulatory protein Vpr., Wecker K, Roques BP, Eur J Biochem. 1999 Dec;266(2):359-69. PMID:10561576
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