MRNA capping enzyme

Function

mRNA capping enzyme (Mce) is a multfunctonal protein with RNA triphosphatase, RNA guanylyltransferase, RNA methyltransferase and transcription factor activities activities. Mce activity entails the transfer of GMP from GTP to the 5'-diphosphate end of mRNA via a enzyme-(lysyl-GMP) intermediate. In Vaccinia virus the active site is at Lys260 within the conserved motif KxDG in thencatalytic subunit^[1].

Disease

Relevance

Structural highlights

3D structures of mRNA capping enzyme3D structures of mRNA capping enzyme

2c46 - hMce TPase domain 1-219 - human
3s24 - hMce GTase domain 229-567
1i9t, 1i9s - mMce TPase domain - mouse
3rtx - mMce GTase domain + DNA-dependent RNA polymerase II C-terminal + guanine
3kyh - yMce α+β subunits - yeast
4pz7 - fyMce α subunit - fission yeast
4pz8 - fyMce α subunit + SPT5
4pz6 - fyMce α subunit + DNA-dependent RNA polymerase II C terminal + GMP
4pn0 - fyMce β subunit
4pn1 - fyMce β subunit + SPT5 peptide
1p16 - Mce α subunit + GTP + RNA polymerase peptide - Candida albicans
1ri5 - EcMce - Encephalitozoon cuniculi
1ri4 - EcMce + SAM
1z3c - EcMce + SAM derivative
1ri3 - EcMce + SAH
1ri2 - EcMce + GTG
1ri1 - EcMce + SAH + GTG
1ckn, 1ckm - PbcvMce + GTP - Paramecium bursaria chlorella virus
1cko - PbcvMce + GPPPG
4cke, 4ckc, 2vdw - VvMce catalytic+regulatory subunits + SAH - Vaccinia virus
4ckb - VvMce catalytic+regulatory subunits + GTP + SAH
6rfl - VvMce catalytic+regulatory subunits + DNA-dependent RNA polymerase - Cryo EM
6rie - VvMce catalytic+regulatory subunits + DNA-dependent RNA polymerase + GDP + SAM - Cryo EM
5x6x - RdvMce - Rice dwarf virus
5x6z - RdvMce + GTP + GDP
5x71 - RdvMce + 5GP
5x6y - RdvMce + SAM
5x70 - RdvMce + RNA

ReferencesReferences

↑ Cong P, Shuman S. Mutational analysis of mRNA capping enzyme identifies amino acids involved in GTP binding, enzyme-guanylate formation, and GMP transfer to RNA. Mol Cell Biol. 1995 Nov;15(11):6222-31. doi: 10.1128/mcb.15.11.6222. PMID:7565775 doi:http://dx.doi.org/10.1128/mcb.15.11.6222

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Cong P, Shuman S. Mutational analysis of mRNA capping enzyme identifies amino acids involved in GTP binding, enzyme-guanylate formation, and GMP transfer to RNA. Mol Cell Biol. 1995 Nov;15(11):6222-31. doi: 10.1128/mcb.15.11.6222. PMID:7565775 doi:http://dx.doi.org/10.1128/mcb.15.11.6222

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