6nhv

Single particle reconstruction of DARPin and its bound GFP on a symmetric scaffoldSingle particle reconstruction of DARPin and its bound GFP on a symmetric scaffold

6nhv, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Structural highlights 6nhv is a 7 chain structure with sequence from Aeqvi, Pseae and Pyrococcus horikoshii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: PA1966 (PSEAE) Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Proteins smaller than about 50 kDa are currently too small to be imaged at high resolution by cryo-electron microscopy (cryo-EM), leaving most protein molecules in the cell beyond the reach of this powerful structural technique. Here we use a designed protein scaffold to bind and symmetrically display 12 copies of a small 26 kDa protein, green fluorescent protein (GFP). We show that the bound cargo protein is held rigidly enough to visualize it at a resolution of 3.8 A by cryo-EM, where specific structural features of the protein are visible. The designed scaffold is modular and can be modified through modest changes in its amino acid sequence to bind and display diverse proteins for imaging, thus providing a general method to break through the lower size limitation in cryo-EM. A 3.8 A resolution cryo-EM structure of a small protein bound to an imaging scaffold.,Liu Y, Huynh DT, Yeates TO Nat Commun. 2019 Apr 23;10(1):1864. doi: 10.1038/s41467-019-09836-0. PMID:31015551 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.