1hbh
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STRUCTURE OF DEOXYHAEMOGLOBIN OF THE ANTARCTIC FISH PAGOTHENIA BERNACCHII AND STRUCTURAL BASIS OF THE ROOT EFFECT
OverviewOverview
We have determined the structure of deoxyhaemoglobin from the antarctic, fish Pagothenia bernacchii at pH 6.2 to a resolution of 2.2 A with X-ray, data from a twinned crystal deconvoluted so as to approximate data from a, single crystal. The R-factor between the (twinned) model and the observed, data is 16% for reflections used in refinement and 22% for reflections not, used in refinement. The T (deoxy) structure was compared with the R, (liganded) structure at pH 8.0 in an attempt to understand the structural, basis of the greater affinity for hydrogen ions of T, relative to R, that, comprises the Root effect. Up to half of the effect can be attributed to, interaction of the residues Asp95 (G1)alpha and Asp101 (G3)beta: in R the, residues are far apart and their carboxyl groups are unprotonated, but the, shift at the alpha 1 beta 2 interface that accompanies the R to T, transition brings them so close that they appear to share a proton between, them. The proximity of Asp99 (G1)beta may contribute to the required, raising of the pKa values of the other two Asp residues. These and, neighbouring residues are sufficiently conserved in the haemoglobins of, trout (component IV), carp and bluefin tuna, all of which exhibit the Root, effect, for the same mechanism to apply. However, the environment is, equally conserved in haemoglobins of Trematomus newnesi (major component), and trout (component I), which do not exhibit the Root effect, so that the, structural factors controlling the Asp-Asp interaction remain unclear. No, other residue appears to undergo an R to T change in the immediate, neighbourhoods that could account for any significant portion of the Root, effect, so at least half of the effect must result either from long-range, electrostatic interactions or from a large number of local interactions.
About this StructureAbout this Structure
1HBH is a Protein complex structure of sequences from Trematomus bernacchii with ACE and HEM as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Structure of deoxyhaemoglobin of the antarctic fish Pagothenia bernacchii with an analysis of the structural basis of the root effect by comparison of the liganded and unliganded haemoglobin structures., Ito N, Komiyama NH, Fermi G, J Mol Biol. 1995 Jul 28;250(5):648-58. PMID:7623382
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