6sy9

Structure of the Legionella pneumophila response regulator LqsRStructure of the Legionella pneumophila response regulator LqsR

Structural highlights 6sy9 is a 1 chain structure with sequence from Atcc 33152. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: C3926_14995, C3927_13800, D7214_05345, ERS240541_01816, ERS253249_00465, NCTC12000_02932 (ATCC 33152) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The water-borne bacterium Legionella pneumophila replicates in environmental protozoa and upon inhalation destroys alveolar macrophages, thus causing a potentially fatal pneumonia termed 'Legionnaires' disease'. L. pneumophila employs the Legionella quorum sensing (Lqs) system to control its life cycle, pathogen-host cell interactions, motility and natural competence. Signaling through the Lqs system occurs through the alpha-hydroxyketone compound Legionella autoinducer-1 (LAI-1) and converges on the prototypic response regulator LqsR, which dimerizes upon phosphorylation of the conserved aspartate, D108 . In this study, we determine the high-resolution structure of monomeric LqsR. The structure reveals a receiver domain adopting a canonical (betaalpha)5 fold, which is connected through an additional sixth helix and an extended alpha5-helix to a novel output domain. The two domains delineate a mainly positively charged groove, and the output domain adopts a five-stranded antiparallel beta-sheet fold similar to nucleotide-binding proteins. Structure-based mutagenesis identified amino acids critical for LqsR phosphorylation and dimerization. Upon phosphorylation, the LqsRD172A and LqsRD302N/E303Q mutant proteins dimerized even more readily than wild-type LqsR, and no evidence for semi-phosphorylated heterodimers was obtained. Taken together, the high-resolution structure of LqsR reveals functionally relevant amino acid residues implicated in signal transduction of the prototypic response regulator. The structure of the Legionella response regulator LqsR reveals amino acids critical for phosphorylation and dimerization.,Hochstrasser R, Hutter CAJ, Arnold FM, Barlocher K, Seeger MA, Hilbi H Mol Microbiol. 2020 Jan 29. doi: 10.1111/mmi.14477. PMID:31997467 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 6sy9, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image