Proteinase-K Pre-Surface Acoustic WaveProteinase-K Pre-Surface Acoustic Wave

Structural highlights

4wob is a 1 chain structure with sequence from Beauveria alba. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:PROK (Beauveria alba)
Activity:Peptidase K, with EC number 3.4.21.64
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues.

Publication Abstract from PubMed

Advances in modern X-ray sources and detector technology have made it possible for crystallographers to collect usable data on crystals of only a few micrometers or less in size. Despite these developments, sample handling techniques have significantly lagged behind and often prevent the full realization of current beamline capabilities. In order to address this shortcoming, a surface acoustic wave-based method for manipulating and patterning crystals is developed. This method, which does not damage the fragile protein crystals, can precisely manipulate and pattern micrometer and submicrometer-sized crystals for data collection and screening. The technique is robust, inexpensive, and easy to implement. This method not only promises to significantly increase efficiency and throughput of both conventional and serial crystallography experiments, but will also make it possible to collect data on samples that were previously intractable.

Precise Manipulation and Patterning of Protein Crystals for Macromolecular Crystallography Using Surface Acoustic Waves.,Guo F, Zhou W, Li P, Mao Z, Yennawar NH, French JB, Huang TJ Small. 2015 Feb 1. doi: 10.1002/smll.201403262. PMID:25641793[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. Guo F, Zhou W, Li P, Mao Z, Yennawar NH, French JB, Huang TJ. Precise Manipulation and Patterning of Protein Crystals for Macromolecular Crystallography Using Surface Acoustic Waves. Small. 2015 Feb 1. doi: 10.1002/smll.201403262. PMID:25641793 doi:http://dx.doi.org/10.1002/smll.201403262

4wob, resolution 1.90Å

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