2c7g
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FPRA FROM MYCOBACTERIUM TUBERCULOSIS: HIS57GLN MUTANT
OverviewOverview
Mycobacterium tuberculosis FprA is a NADPH-ferredoxin reductase, functionally and structurally similar to the mammalian adrenodoxin, reductase. It is presumably involved in supplying electrons to one or more, of the pathogen's cytochrome P450s through reduced ferredoxins. It has, been proposed on the basis of crystallographic data (Bossi, R. T., et al., (2002) Biochemistry 41, 8807-8818) that the highly conserved His57 and, Glu214 whose side chains are H-bonded are involved in catalysis. Both, residues were individually changed to nonionizable amino acyl residues, through site-directed mutagenesis. Steady-state kinetics showed that the, role of Glu214 in catalysis is negligible. On the contrary, the, substitutions of His57 markedly impaired the catalytic efficiency of FprA, for ferredoxin in the physiological reaction. Furthemore, they decreased, the k(cat)/K(m) value for NADPH in the ferricyanide reduction., Rapid-reaction (stopped-flow) kinetic analysis of the isolated reductive, half-reaction of wild-type and His57Gln forms of FprA with NADPH and NADH, allowed a detailed description of the mechanism of enzyme-bound FAD, reduction, with the identification of the intermediates involved. The, His57Gln mutation caused a 6-fold decrease in the rate of hydride transfer, from either NADPH or NADH to the enzyme-bound FAD cofactor. The 3D, structure of FprA-H57Q, obtained at 1.8 A resolution, explains the, inefficient hydride transfer of the mutant in terms of a suboptimal, geometry of the nicotinamide-isoalloxazine interaction in the active site., These data demonstrate the role of His57 in the correct binding of NADPH, to FprA for the subsequent steps of the catalytic cycle to proceed at a, high rate.
About this StructureAbout this Structure
2C7G is a Single protein structure of sequence from Mycobacterium tuberculosis with NA, FAD and ODP as ligands. Active as Ferredoxin--NADP(+) reductase, with EC number 1.18.1.2 Structure known Active Site: AC1. Full crystallographic information is available from OCA.
ReferenceReference
Role of the His57-Glu214 ionic couple located in the active site of Mycobacterium tuberculosis FprA., Pennati A, Razeto A, de Rosa M, Pandini V, Vanoni MA, Mattevi A, Coda A, Aliverti A, Zanetti G, Biochemistry. 2006 Jul 25;45(29):8712-20. PMID:16846214
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