2gjw
RNA Recognition and Cleavage by an Splicing Endonuclease
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| , resolution 2.85Å | |||||||
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| Ligands: | , , , , , | ||||||
| Gene: | endA (Archaeoglobus fulgidus) | ||||||
| Activity: | tRNA-intron endonuclease, with EC number 3.1.27.9 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
OverviewOverview
The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a cleaved splice site. The endonuclease dimer cooperatively recognized a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line RNA cleavage reaction. Cooperativity arises because an arginine pair from one catalytic domain sandwiches a nucleobase within the bulge cleaved by the other catalytic domain.
About this StructureAbout this Structure
2GJW is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
ReferenceReference
RNA recognition and cleavage by a splicing endonuclease., Xue S, Calvin K, Li H, Science. 2006 May 12;312(5775):906-10. PMID:16690865
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